Anti-inflammatory, antinociceptive and anti-angiogenic activities of a phospholipid mixture purified from porcine lung tissues

Hyun Joo Jung, Jeong Su Moon, A. Rum Park, Hojin Choi, Jong Eun Lee, Seong Hyun Choi, Chang Jin Lim

Research output: Contribution to journalArticle

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Abstract

This work aimed to assess anti-inflammatory and related properties of a phospholipid mixture purified from porcine lung tissues, named KT&G101, which is being developed as a novel topical remedy for atopic dermatitis. KT&G101 consists of pure phospholipids, mainly phosphatidylcholine (PC) and other phospholipids such as phosphatidylinositol (PI) and phosphatidylserine (PS). Its predominant PC species is 1,2-dipalmitoylphosphatidylcholine (DPPC). KT&G101 exhibited an anti-angiogenic activity in the chick chorioallantoic membrane (CAM) assay. Oral administration of KT&G101 at the dosages of 100, 200 and 400mg/kg body weight gave rise to an inhibition of 15.4%, 25.3% and 30.1% in the vascular permeability assay, respectively. In the carrageenan-induced inflammation in the air pouches, KT&G101 significantly diminished the volume of exudates in the pouches, the number of polymorphonuclear leukocytes and nitrite content in exudates. In the acetic acid-induced writhing response, oral administration of KT&G101 at the dosages of 50, 100 and 200mg/kg body weight showed the reduction of 21.6%, 51.6% and 60.8% in the pain response of mice, respectively. It was also able to diminish the nitric oxide (NO) and reactive oxygen species (ROS) levels in the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. KT&G101 displayed a significant suppression on the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in the stimulated RAW264.7 cells. However, the free radical scavenging activity of KT&G101 was detected to be very weak in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Taken together, KT&G101 possesses anti-inflammatory and related antinociceptive and anti-angiogenic activities, which indirectly supports its use as an anti-atopic therapy.

Original languageEnglish
Pages (from-to)398-407
Number of pages10
JournalImmunopharmacology and Immunotoxicology
Volume34
Issue number3
DOIs
Publication statusPublished - 2012 Jun 1

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Assays
Phospholipids
Anti-Inflammatory Agents
Swine
Exudates and Transudates
Tissue
Phosphatidylcholines
Lung
Oral Administration
Body Weight
Chorioallantoic Membrane
1,2-Dipalmitoylphosphatidylcholine
Macrophages
Carrageenan
Phosphatidylserines
Scavenging
Capillary Permeability
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Atopic Dermatitis

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Toxicology
  • Pharmacology

Cite this

Jung, Hyun Joo ; Moon, Jeong Su ; Park, A. Rum ; Choi, Hojin ; Lee, Jong Eun ; Choi, Seong Hyun ; Lim, Chang Jin. / Anti-inflammatory, antinociceptive and anti-angiogenic activities of a phospholipid mixture purified from porcine lung tissues. In: Immunopharmacology and Immunotoxicology. 2012 ; Vol. 34, No. 3. pp. 398-407.
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abstract = "This work aimed to assess anti-inflammatory and related properties of a phospholipid mixture purified from porcine lung tissues, named KT&G101, which is being developed as a novel topical remedy for atopic dermatitis. KT&G101 consists of pure phospholipids, mainly phosphatidylcholine (PC) and other phospholipids such as phosphatidylinositol (PI) and phosphatidylserine (PS). Its predominant PC species is 1,2-dipalmitoylphosphatidylcholine (DPPC). KT&G101 exhibited an anti-angiogenic activity in the chick chorioallantoic membrane (CAM) assay. Oral administration of KT&G101 at the dosages of 100, 200 and 400mg/kg body weight gave rise to an inhibition of 15.4{\%}, 25.3{\%} and 30.1{\%} in the vascular permeability assay, respectively. In the carrageenan-induced inflammation in the air pouches, KT&G101 significantly diminished the volume of exudates in the pouches, the number of polymorphonuclear leukocytes and nitrite content in exudates. In the acetic acid-induced writhing response, oral administration of KT&G101 at the dosages of 50, 100 and 200mg/kg body weight showed the reduction of 21.6{\%}, 51.6{\%} and 60.8{\%} in the pain response of mice, respectively. It was also able to diminish the nitric oxide (NO) and reactive oxygen species (ROS) levels in the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. KT&G101 displayed a significant suppression on the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in the stimulated RAW264.7 cells. However, the free radical scavenging activity of KT&G101 was detected to be very weak in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Taken together, KT&G101 possesses anti-inflammatory and related antinociceptive and anti-angiogenic activities, which indirectly supports its use as an anti-atopic therapy.",
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Anti-inflammatory, antinociceptive and anti-angiogenic activities of a phospholipid mixture purified from porcine lung tissues. / Jung, Hyun Joo; Moon, Jeong Su; Park, A. Rum; Choi, Hojin; Lee, Jong Eun; Choi, Seong Hyun; Lim, Chang Jin.

In: Immunopharmacology and Immunotoxicology, Vol. 34, No. 3, 01.06.2012, p. 398-407.

Research output: Contribution to journalArticle

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AU - Jung, Hyun Joo

AU - Moon, Jeong Su

AU - Park, A. Rum

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AU - Lee, Jong Eun

AU - Choi, Seong Hyun

AU - Lim, Chang Jin

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