Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP kinase

Jung Eun Lee, Soo Muk Cho, Eunkyo Park, Seung Min Lee, Yuri Kim, Joong Hyuck Auh, Hyung Kyoon Choi, Sohee Lim, Sung Chul Lee, Jung Hyun Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

MATERIALS/METHODS: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-1β, and IL-6) and NO and PGE2 productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-κB and MAPK activities.

RESULTS: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions. RE and RH treatments reduced IL-1β and IL-6 expressions through suppressions of JNK and p38 phosphorylation.

CONCLUSIONS: In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.

BACKGROUND/OBJECTIVES: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells.

Original languageEnglish
Pages (from-to)501-508
Number of pages8
JournalNutrition Research and Practice
Volume8
Issue number5
DOIs
Publication statusPublished - 2014 Oct 1

Fingerprint

Rubus coreanus
anti-inflammatory activity
mitogen-activated protein kinase
Anti-Inflammatory Agents
Phosphotransferases
extracts
Ethanol
ethanol
interleukin-1
prostaglandin synthase
Interleukin-1
Dinoprostone
interleukin-6
Interleukin-6
phosphorylation
Korean Traditional Medicine
inflammation
Phosphorylation
Rubus
rheumatoid arthritis

All Science Journal Classification (ASJC) codes

  • Food Science
  • Nutrition and Dietetics

Cite this

Lee, Jung Eun ; Cho, Soo Muk ; Park, Eunkyo ; Lee, Seung Min ; Kim, Yuri ; Auh, Joong Hyuck ; Choi, Hyung Kyoon ; Lim, Sohee ; Lee, Sung Chul ; Kim, Jung Hyun. / Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP kinase. In: Nutrition Research and Practice. 2014 ; Vol. 8, No. 5. pp. 501-508.
@article{804d5a6ad82d422db3c1e9e45e427417,
title = "Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP kinase",
abstract = "MATERIALS/METHODS: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-1β, and IL-6) and NO and PGE2 productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-κB and MAPK activities.RESULTS: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions. RE and RH treatments reduced IL-1β and IL-6 expressions through suppressions of JNK and p38 phosphorylation.CONCLUSIONS: In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.BACKGROUND/OBJECTIVES: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells.",
author = "Lee, {Jung Eun} and Cho, {Soo Muk} and Eunkyo Park and Lee, {Seung Min} and Yuri Kim and Auh, {Joong Hyuck} and Choi, {Hyung Kyoon} and Sohee Lim and Lee, {Sung Chul} and Kim, {Jung Hyun}",
year = "2014",
month = "10",
day = "1",
doi = "10.4162/nrp.2014.8.5.501",
language = "English",
volume = "8",
pages = "501--508",
journal = "Nutrition Research and Practice",
issn = "1976-1457",
publisher = "The Korean Society of Community Nutrition",
number = "5",

}

Lee, JE, Cho, SM, Park, E, Lee, SM, Kim, Y, Auh, JH, Choi, HK, Lim, S, Lee, SC & Kim, JH 2014, 'Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP kinase', Nutrition Research and Practice, vol. 8, no. 5, pp. 501-508. https://doi.org/10.4162/nrp.2014.8.5.501

Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP kinase. / Lee, Jung Eun; Cho, Soo Muk; Park, Eunkyo; Lee, Seung Min; Kim, Yuri; Auh, Joong Hyuck; Choi, Hyung Kyoon; Lim, Sohee; Lee, Sung Chul; Kim, Jung Hyun.

In: Nutrition Research and Practice, Vol. 8, No. 5, 01.10.2014, p. 501-508.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-κB and MAP kinase

AU - Lee, Jung Eun

AU - Cho, Soo Muk

AU - Park, Eunkyo

AU - Lee, Seung Min

AU - Kim, Yuri

AU - Auh, Joong Hyuck

AU - Choi, Hyung Kyoon

AU - Lim, Sohee

AU - Lee, Sung Chul

AU - Kim, Jung Hyun

PY - 2014/10/1

Y1 - 2014/10/1

N2 - MATERIALS/METHODS: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-1β, and IL-6) and NO and PGE2 productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-κB and MAPK activities.RESULTS: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions. RE and RH treatments reduced IL-1β and IL-6 expressions through suppressions of JNK and p38 phosphorylation.CONCLUSIONS: In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.BACKGROUND/OBJECTIVES: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells.

AB - MATERIALS/METHODS: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-α, IL-1β, and IL-6) and NO and PGE2 productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-κB and MAPK activities.RESULTS: UE and UH treatment significantly reduced NF-κB activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and PGE2 productions. RE and RH treatments reduced IL-1β and IL-6 expressions through suppressions of JNK and p38 phosphorylation.CONCLUSIONS: In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-κB and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.BACKGROUND/OBJECTIVES: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells.

UR - http://www.scopus.com/inward/record.url?scp=84908071632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908071632&partnerID=8YFLogxK

U2 - 10.4162/nrp.2014.8.5.501

DO - 10.4162/nrp.2014.8.5.501

M3 - Article

AN - SCOPUS:84908071632

VL - 8

SP - 501

EP - 508

JO - Nutrition Research and Practice

JF - Nutrition Research and Practice

SN - 1976-1457

IS - 5

ER -