Anti-leukemia activity of a Hsp70 inhibitor and its hybrid molecules

Seong Hyun Park, Won Je Kim, Hui Li, Wonil Seo, Sang Hyun Park, Hwan Kim, Sang Chul Shin, Erik R.P. Zuiderweg, Eunice Eun Kyeong Kim, Taebo Sim, Nak Kyoon Kim, Injae Shin

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Abstract

In this study we examined the anti-leukemia activity of a small molecule inhibitor of Hsp70 proteins, apoptozole (Az), and hybrids in which it is linked to an inhibitor of either Hsp90 (geldanamycin) or Abl kinase (imatinib). The results of NMR studies revealed that Az associates with an ATPase domain of Hsc70 and thus blocks ATP binding to the protein. Observations made in the cell study indicated that Az treatment promotes leukemia cell death by activating caspase-dependent apoptosis without affecting the caspase-independent apoptotic pathway. Importantly, the hybrids composed of Az and geldanamycin, which have high inhibitory activities towards both Hsp70 and Hsp90, exhibit enhanced anti-leukemia activity relative to the individual inhibitors. However, the Az and imatinib hybrids have weak inhibitory activities towards Hsp70 and Abl, and display lower cytotoxicity against leukemia cells compared to those of the individual constituents. The results of a mechanistic study showed that the active hybrid molecules promote leukemia cell death through a caspase-dependent apoptotic pathway. Taken together, the findings suggest that Hsp70 inhibitors as well as their hybrids can serve as potential anti-leukemia agents.

Original languageEnglish
Article number3537
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Leukemia
Caspases
Cell Death
Adenosine Triphosphatases
Carrier Proteins
Phosphotransferases
Adenosine Triphosphate
apoptozole
Apoptosis
Proteins
Imatinib Mesylate
geldanamycin

All Science Journal Classification (ASJC) codes

  • General

Cite this

Park, Seong Hyun ; Kim, Won Je ; Li, Hui ; Seo, Wonil ; Park, Sang Hyun ; Kim, Hwan ; Shin, Sang Chul ; Zuiderweg, Erik R.P. ; Kim, Eunice Eun Kyeong ; Sim, Taebo ; Kim, Nak Kyoon ; Shin, Injae. / Anti-leukemia activity of a Hsp70 inhibitor and its hybrid molecules. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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abstract = "In this study we examined the anti-leukemia activity of a small molecule inhibitor of Hsp70 proteins, apoptozole (Az), and hybrids in which it is linked to an inhibitor of either Hsp90 (geldanamycin) or Abl kinase (imatinib). The results of NMR studies revealed that Az associates with an ATPase domain of Hsc70 and thus blocks ATP binding to the protein. Observations made in the cell study indicated that Az treatment promotes leukemia cell death by activating caspase-dependent apoptosis without affecting the caspase-independent apoptotic pathway. Importantly, the hybrids composed of Az and geldanamycin, which have high inhibitory activities towards both Hsp70 and Hsp90, exhibit enhanced anti-leukemia activity relative to the individual inhibitors. However, the Az and imatinib hybrids have weak inhibitory activities towards Hsp70 and Abl, and display lower cytotoxicity against leukemia cells compared to those of the individual constituents. The results of a mechanistic study showed that the active hybrid molecules promote leukemia cell death through a caspase-dependent apoptotic pathway. Taken together, the findings suggest that Hsp70 inhibitors as well as their hybrids can serve as potential anti-leukemia agents.",
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Park, SH, Kim, WJ, Li, H, Seo, W, Park, SH, Kim, H, Shin, SC, Zuiderweg, ERP, Kim, EEK, Sim, T, Kim, NK & Shin, I 2017, 'Anti-leukemia activity of a Hsp70 inhibitor and its hybrid molecules', Scientific reports, vol. 7, no. 1, 3537. https://doi.org/10.1038/s41598-017-03814-6

Anti-leukemia activity of a Hsp70 inhibitor and its hybrid molecules. / Park, Seong Hyun; Kim, Won Je; Li, Hui; Seo, Wonil; Park, Sang Hyun; Kim, Hwan; Shin, Sang Chul; Zuiderweg, Erik R.P.; Kim, Eunice Eun Kyeong; Sim, Taebo; Kim, Nak Kyoon; Shin, Injae.

In: Scientific reports, Vol. 7, No. 1, 3537, 01.12.2017.

Research output: Contribution to journalArticle

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AU - Kim, Won Je

AU - Li, Hui

AU - Seo, Wonil

AU - Park, Sang Hyun

AU - Kim, Hwan

AU - Shin, Sang Chul

AU - Zuiderweg, Erik R.P.

AU - Kim, Eunice Eun Kyeong

AU - Sim, Taebo

AU - Kim, Nak Kyoon

AU - Shin, Injae

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