Anti-oxidant and anti-inflammatory activities of macelignan in murine hippocampal cell line and primary culture of rat microglial cells

Da Qing Jin, Chol Seung Lim, Jae Kwan Hwang, Ilho Ha, Jung Soo Han

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Epidemiological studies suggest that the treatments of anti-inflammatory agents and anti-oxidants slow the progress of neurological diseases. Lignans are anti-oxidants and phytoestrogens found in a variety of plants. In this study, we investigated the neuroprotective effect of macelignan on glutamate-induced neurotoxicity and reactive oxygen species (ROS) in murine hippocampal HT22 cell line. Macelignan significantly attenuated the ROS production and neurotoxicity induced by glutamate in HT22 cell. Also, the properties of macelignan as an anti-inflammatory agent were investigated in microglials activation by lipopolysaccharide (LPS). It potently suppressed the expression of cyclooxygenase-2 and inducible nitric oxide synthase, that consequently resulted in the reduction of nitric oxide in LPS-treated microglial cells. It also significantly suppressed the production of pro-inflammatory cytokine tumor necrosis factor-α and interleukin-6. These results suggest that macelignan possesses therapeutic potentials against neurodegenerative diseases with oxidative stress and neuroinflammation.

Original languageEnglish
Pages (from-to)1264-1269
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume331
Issue number4
DOIs
Publication statusPublished - 2005 Jun 17

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Cell culture
Oxidants
Rats
Anti-Inflammatory Agents
Cells
Cell Line
Lipopolysaccharides
Glutamic Acid
Reactive Oxygen Species
Neurodegenerative diseases
Phytoestrogens
Lignans
Oxidative stress
Neuroprotective Agents
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Neurodegenerative Diseases
Epidemiologic Studies
Interleukin-6
Nitric Oxide

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Epidemiological studies suggest that the treatments of anti-inflammatory agents and anti-oxidants slow the progress of neurological diseases. Lignans are anti-oxidants and phytoestrogens found in a variety of plants. In this study, we investigated the neuroprotective effect of macelignan on glutamate-induced neurotoxicity and reactive oxygen species (ROS) in murine hippocampal HT22 cell line. Macelignan significantly attenuated the ROS production and neurotoxicity induced by glutamate in HT22 cell. Also, the properties of macelignan as an anti-inflammatory agent were investigated in microglials activation by lipopolysaccharide (LPS). It potently suppressed the expression of cyclooxygenase-2 and inducible nitric oxide synthase, that consequently resulted in the reduction of nitric oxide in LPS-treated microglial cells. It also significantly suppressed the production of pro-inflammatory cytokine tumor necrosis factor-α and interleukin-6. These results suggest that macelignan possesses therapeutic potentials against neurodegenerative diseases with oxidative stress and neuroinflammation.",
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Anti-oxidant and anti-inflammatory activities of macelignan in murine hippocampal cell line and primary culture of rat microglial cells. / Jin, Da Qing; Lim, Chol Seung; Hwang, Jae Kwan; Ha, Ilho; Han, Jung Soo.

In: Biochemical and Biophysical Research Communications, Vol. 331, No. 4, 17.06.2005, p. 1264-1269.

Research output: Contribution to journalArticle

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