Abstract
Neospora caninum is an apicomplexan parasite associated with abortion in cattle worldwide. Apicidin, histone deacetylase (HDAC) inhibitor, has shown a broad spectrum of anti-protozoal activity against apicomplexan parasites. Cultured vero cells infected with N. caninum tachyzoites were treated with 0.5μg/ml of depudecin, another known natural product isolated from Altermaria brassiciicola with anti-histone deacetylase activity, to examine the efficacy of depudecin against intracellular multiplication of N. caninum tachyzoite, using 15ng/ml of apicidin as the control. Depudecin significantly inhibited the intracellular multiplication of N. caninum at a level similar to that of apicidin without exerting any cytotoxicity on the host vero cells. Acid/urea/Triton gel electrophoresis analysis show anti-parasitic activity of depudecin appears to be due to the inhibition of protozoal histone deacetylase, which induces hyperacetylation of histones in N. caninum.
| Original language | English |
|---|---|
| Pages (from-to) | 269-276 |
| Number of pages | 8 |
| Journal | Veterinary Parasitology |
| Volume | 112 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2003 Mar 25 |
Bibliographical note
Funding Information:This work was supported by grants from the Korea Science & Engineering Foundation No. R01-1999-00121 to H.J.K., the Brain Korea 21 Project to D.-Y.K., and the Ministry of Agriculture and Forestry (399002-3).
All Science Journal Classification (ASJC) codes
- Parasitology
- veterinary(all)