Anti-skeletal muscle atrophy effect of Oenothera odorata root extract via reactive oxygen species-dependent signaling pathways in cellular and mouse model

Yong Hyeon Lee, Wan Joong Kim, Myung Hun Lee, Sun Young Kim, Dong Hyun Seo, Han Sung Kim, Michael Gelinsky, Tack Joong Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Skeletal muscle atrophy can be defined as a decrease of muscle volume caused by injury or lack of use. This condition is associated with reactive oxygen species (ROS), resulting in various muscular disorders. We acquired 2D and 3D images using micro-computed tomography in gastrocnemius and soleus muscles of sciatic-denervated mice. We confirmed that sciatic denervation-small animal model reduced muscle volume. However, the intraperitoneal injection of Oenothera odorata root extract (EVP) delayed muscle atrophy compared to a control group. We also investigated the mechanism of muscle atrophy's relationship with ROS. EVP suppressed expression of SOD1, and increased expression of HSP70, in both H2O2-treated C2C12 myoblasts and sciatic-denervated mice. Moreover, EVP regulated apoptotic signals, including caspase- 3, Bax, Bcl-2, and ceramide. These results indicate that EVP has a positive effect on reducing the effect of ROS on muscle atrophy.

Original languageEnglish
Pages (from-to)80-88
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Oenothera
Muscular Atrophy
Muscle
Reactive Oxygen Species
Skeletal Muscle
Muscles
Myoblasts
Ceramides
Denervation
Intraperitoneal Injections
Caspase 3
Animal Models
Tomography
Control Groups
Wounds and Injuries
Animals

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Lee, Yong Hyeon ; Kim, Wan Joong ; Lee, Myung Hun ; Kim, Sun Young ; Seo, Dong Hyun ; Kim, Han Sung ; Gelinsky, Michael ; Kim, Tack Joong. / Anti-skeletal muscle atrophy effect of Oenothera odorata root extract via reactive oxygen species-dependent signaling pathways in cellular and mouse model. In: Bioscience, Biotechnology and Biochemistry. 2016 ; Vol. 80, No. 1. pp. 80-88.
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Anti-skeletal muscle atrophy effect of Oenothera odorata root extract via reactive oxygen species-dependent signaling pathways in cellular and mouse model. / Lee, Yong Hyeon; Kim, Wan Joong; Lee, Myung Hun; Kim, Sun Young; Seo, Dong Hyun; Kim, Han Sung; Gelinsky, Michael; Kim, Tack Joong.

In: Bioscience, Biotechnology and Biochemistry, Vol. 80, No. 1, 01.01.2016, p. 80-88.

Research output: Contribution to journalArticle

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