Anti-T antibodies and peanut-agglutinin-binding glycoproteins in sera of patients with gastric cancer

II Ran Hwang, Dong Ho Nahm, Sang Nae Cho, B. Michael Longenecker, R. Rao Koganty, In Suh Park

Research output: Contribution to journalArticle

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Abstract

Agglutinating antibodies to neuraminidase-treated red blood cells (anti- T agglutinins) are known to be reduced in patients with gastric cancer. The antigenic determinant of anti-T agglutinin is known to have a disaccharide structure [Gal(β1-3)GalNAc], the same specificity as peanut agglutinin (PNA). We examined sera of 27 patients with gastric cancer and 30 controls for anti-T agglutinins, anti-T antibodies and PNA-binding glycoproteins. Anti-T agglutinins were titrated by a microtiter hemagglutination method. Levels of anti-T antibodies were determined by enzyme immunoassay using synthetic glycoconjugate [Gal(β1-3)GalNAc O-α-linked to human serum albumin] as an antigen. Levels of PNA-binding glycoproteins in sera were measured by sandwich enzyme-linked lectin assay using wheat germ agglutinin and peroxidase-conjugated PNA. Titers of anti-T agglutinins were significantly lower in patients with gastric cancer than in controls (P = 0.041). Levels of anti-T antibodies were not significantly different in patients with gastric cancer and controls; however, decreased levels of anti- T antibodies were more frequent in patients with gastric cancer than in controls (P = 0.001). Levels of PNA-binding glycoproteins were significantly higher in sera of patients with gastric cancer than in controls (P = 0.001). The levels of anti-T antibodies inversely correlated with the levels of PNA- binding glycoproteins in sera of patients with gastric cancer (r = -0.44, P = 0.021). These results suggest that the decrease in anti-T antibodies in sera of patients with gastric cancer might be due to immune complex formation between circulating PNA-binding glycoproteins and anti-T antibodies.

Original languageEnglish
Pages (from-to)582-587
Number of pages6
JournalJournal of cancer research and clinical oncology
Volume125
Issue number10
DOIs
Publication statusPublished - 1999 Sep 24

Fingerprint

Peanut Agglutinin
Stomach Neoplasms
Anti-Idiotypic Antibodies
Glycoproteins
Serum
Thomsen-Friedenreich antibodies
Wheat Germ Agglutinins
Glycoconjugates
Disaccharides
Hemagglutination
Neuraminidase
Antigen-Antibody Complex
Immunoenzyme Techniques
Lectins
Serum Albumin
Peroxidase
T-agglutinin
Epitopes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Hwang, II Ran ; Nahm, Dong Ho ; Cho, Sang Nae ; Longenecker, B. Michael ; Koganty, R. Rao ; Park, In Suh. / Anti-T antibodies and peanut-agglutinin-binding glycoproteins in sera of patients with gastric cancer. In: Journal of cancer research and clinical oncology. 1999 ; Vol. 125, No. 10. pp. 582-587.
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abstract = "Agglutinating antibodies to neuraminidase-treated red blood cells (anti- T agglutinins) are known to be reduced in patients with gastric cancer. The antigenic determinant of anti-T agglutinin is known to have a disaccharide structure [Gal(β1-3)GalNAc], the same specificity as peanut agglutinin (PNA). We examined sera of 27 patients with gastric cancer and 30 controls for anti-T agglutinins, anti-T antibodies and PNA-binding glycoproteins. Anti-T agglutinins were titrated by a microtiter hemagglutination method. Levels of anti-T antibodies were determined by enzyme immunoassay using synthetic glycoconjugate [Gal(β1-3)GalNAc O-α-linked to human serum albumin] as an antigen. Levels of PNA-binding glycoproteins in sera were measured by sandwich enzyme-linked lectin assay using wheat germ agglutinin and peroxidase-conjugated PNA. Titers of anti-T agglutinins were significantly lower in patients with gastric cancer than in controls (P = 0.041). Levels of anti-T antibodies were not significantly different in patients with gastric cancer and controls; however, decreased levels of anti- T antibodies were more frequent in patients with gastric cancer than in controls (P = 0.001). Levels of PNA-binding glycoproteins were significantly higher in sera of patients with gastric cancer than in controls (P = 0.001). The levels of anti-T antibodies inversely correlated with the levels of PNA- binding glycoproteins in sera of patients with gastric cancer (r = -0.44, P = 0.021). These results suggest that the decrease in anti-T antibodies in sera of patients with gastric cancer might be due to immune complex formation between circulating PNA-binding glycoproteins and anti-T antibodies.",
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Anti-T antibodies and peanut-agglutinin-binding glycoproteins in sera of patients with gastric cancer. / Hwang, II Ran; Nahm, Dong Ho; Cho, Sang Nae; Longenecker, B. Michael; Koganty, R. Rao; Park, In Suh.

In: Journal of cancer research and clinical oncology, Vol. 125, No. 10, 24.09.1999, p. 582-587.

Research output: Contribution to journalArticle

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AU - Hwang, II Ran

AU - Nahm, Dong Ho

AU - Cho, Sang Nae

AU - Longenecker, B. Michael

AU - Koganty, R. Rao

AU - Park, In Suh

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