Anti-tumor activity of ex vivo expanded cytokine-induced killer cells against human hepatocellular carcinoma

Hwan Mook Kim, Jaeseung Lim, Yeo Dae Yoon, Ji Mi Ahn, Jong Soon Kang, Kiho Lee, Song Kyu Park, Yu Jin Jeong, Jin Mi Kim, Gyoonhee Han, Kyu Hwan Yang, Yeon Jin Kim, Youngsoo Kim, Sang Bae Han

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40 Citations (Scopus)

Abstract

Cytokine-induced killer (CIK) cells are ex vivo expanded T cells with natural killer cell phenotypes and functions. In this study, the anti-tumor activity of CIK cells against hepatocellular carcinoma was evaluated in vitro and in vivo. In the presence of anti-CD3 antibody and IL-2 for 14 days, human peripheral blood mononuclear cell population changed to heterogeneous CIK cell population, which comprised 96% CD3+, 3% CD3¡©CD56+, 32% CD3+CD56+, 11% CD4+, 75% CD8+, and 30% CD8+CD56+. CIK cells produced significant amounts of IFN-γ and TNF-α; however, produced only slight amounts of IL-2, IL-4, and IL-5. At an effector-target cell ratio of 30:1, CIK cells destroyed 33% of SNU-354 human hepatocellular carcinoma cells, which was determined by the 51Cr-release assay. In addition, a dose of 1 × 106 CIK cells per mouse inhibited 60% of SNU-354 tumor growth in irradiated nude mice. This study suggests that CIK cells may be used as an adoptive immunotherapy for patients with hepatocellular carcinoma.

Original languageEnglish
Pages (from-to)1793-1801
Number of pages9
JournalInternational Immunopharmacology
Volume7
Issue number13
DOIs
Publication statusPublished - 2007 Dec 15

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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    Kim, H. M., Lim, J., Yoon, Y. D., Ahn, J. M., Kang, J. S., Lee, K., Park, S. K., Jeong, Y. J., Kim, J. M., Han, G., Yang, K. H., Kim, Y. J., Kim, Y., & Han, S. B. (2007). Anti-tumor activity of ex vivo expanded cytokine-induced killer cells against human hepatocellular carcinoma. International Immunopharmacology, 7(13), 1793-1801. https://doi.org/10.1016/j.intimp.2007.08.007