Antiangiogenic activity of the lipophilic antimicrobial peptides from an endophytic bacterial strain isolated from red pepper leaf

Hye Jin Jung, Yonghyo Kim, Hyang Burm Lee, Ho Jeong Kwon

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The induction of angiogenesis is a crucial step in tumor progression, and therefore, efficient inhibition of angio-genesis is considered a powerful strategy for the treatment of cancer. In the present study, we report that the lipophilic antimicrobial peptides from EML-CAP3, a new endophytic bacterial strain isolated from red pepper leaf (Capsicum annuum L.), exhibit potent antiangiogenic activity both in vitro and in vivo. The newly obtained antimi-crobial peptides effectively inhibited the proliferation of human umbilical vein endothelial cells at subtoxic doses. Furthermore, the peptides suppressed the in vitro charac-teristics of angiogenesis such as endothelial cell invasion and tube formation stimulated by vascular endothelial growth factor, as well as neovascularization of the chorioallantoic membrane of growing chick embryos in vivo without showing cytotoxicity. Notably, the angiostatic peptides blocked tumor cell-induced angiogenesis by suppressing the expression levels of hypoxia-inducible factor-1α and its target gene, vascular endothelial growth factor (VEGF). To our knowledge, our findings demon-strate for the first time that the antimicrobial peptides from EML-CAP3 possess antiangiogenic potential and may thus be used for the treatment of hypervascularized tumors.

Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalMolecules and cells
Volume38
Issue number3
DOIs
Publication statusPublished - 2015 Mar

Bibliographical note

Funding Information:
This study was partly supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean Government (2010-0017984 and 2012M3A9D1054520), the Translational Research Center for Protein Function Control, KRF (2009-0083522), the Next-Generation BioGreen 21 Program (No.PJ0079772012), the Rural Development Administra-tion, the National R&D Program, the Ministry of Health & Wel-fare (0620360-1), the Bio-industry Technology Development program (111095-3) for IPET, Ministry of Agriculture, Food, and Rural Affairs, the Basic Science Research Program, the Ministry of Education (NRF-2014R1A1A2057902), and the Brain Korea 21 Plus Project, Republic of Korea.

Funding Information:
This study was partly supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean Government (2010-0017984 and 2012M3A9D1054520), the Translational Research Center for Protein Function Control, KRF (2009-0083522), the Next-Generation BioGreen 21 Program (No.PJ0079772012), the Rural Development Administration, the National R&D Program, the Ministry of Health & Welfare (0620360-1), the Bio-industry Technology Development program (111095-3) for IPET, Ministry of Agriculture, Food, and Rural Affairs, the Basic Science Research Program, the Ministry of Education (NRF-2014R1A1A2057902), and the Brain Korea 21 Plus Project, Republic of Korea.

Publisher Copyright:
The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons.

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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