Antibacterial activity and effect on gingival cells of microwave-pulsed non-thermal atmospheric pressure plasma in artificial saliva

Sang Hee Seo, Ihn Han, Han Seol Lee, Jin Joo Choi, Eun Ha Choi, Kyoung Nam Kim, Gyungsoon Park, Kwangmahn Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although various oral pathogens are inactivated by non-thermal atmospheric pressure plasma (NTAPP), the in vivo effects of NTAPP are poorly understood. The first aim of this study was to examine the antibacterial activity of microwave-pulsed NTAPP against Staphylococcus aureus in artificial saliva to mimic oral environmental conditions. The second aim was to determine the influence of microwave-pulsed NTAPP on human gingival fibroblasts (HGFs). The microwave-pulsed NTAPP reduced bacterial viability (as measured by colony forming units [CFU]) to a greater extent in artificial saliva than in saline. Extending the post-treatment incubation time increased bacterial inactivation in artificial saliva compared to saline. HGFs viability was unaffected by microwave-pulsed NTAPP for bacterial inactivation. Rather, HGFs proliferation increased after a 5-min microwave-pulsed NTAPP. Less tumor necrosis factor alpha was released by microwave-pulsed NTAPP-treated HGFs stimulated with lipopolysaccharide (LPS) than by untreated, LPS-stimulated HGFs; thus, plasma appeared to suppress the inflammatory response. Our study suggests that microwave-pulsed NTAPP may have stronger in vivo antibacterial activity than in vitro activity, and that microwave-pulsed NTAPP may have the additional advantage of suppressing gingival inflammatory responses.

Original languageEnglish
Article number8395
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Artificial Saliva
Plasma Gases
Microwaves
Fibroblasts
Lipopolysaccharides
Microbial Viability
Staphylococcus aureus
Stem Cells
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • General

Cite this

Seo, Sang Hee ; Han, Ihn ; Lee, Han Seol ; Choi, Jin Joo ; Choi, Eun Ha ; Kim, Kyoung Nam ; Park, Gyungsoon ; Kim, Kwangmahn. / Antibacterial activity and effect on gingival cells of microwave-pulsed non-thermal atmospheric pressure plasma in artificial saliva. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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abstract = "Although various oral pathogens are inactivated by non-thermal atmospheric pressure plasma (NTAPP), the in vivo effects of NTAPP are poorly understood. The first aim of this study was to examine the antibacterial activity of microwave-pulsed NTAPP against Staphylococcus aureus in artificial saliva to mimic oral environmental conditions. The second aim was to determine the influence of microwave-pulsed NTAPP on human gingival fibroblasts (HGFs). The microwave-pulsed NTAPP reduced bacterial viability (as measured by colony forming units [CFU]) to a greater extent in artificial saliva than in saline. Extending the post-treatment incubation time increased bacterial inactivation in artificial saliva compared to saline. HGFs viability was unaffected by microwave-pulsed NTAPP for bacterial inactivation. Rather, HGFs proliferation increased after a 5-min microwave-pulsed NTAPP. Less tumor necrosis factor alpha was released by microwave-pulsed NTAPP-treated HGFs stimulated with lipopolysaccharide (LPS) than by untreated, LPS-stimulated HGFs; thus, plasma appeared to suppress the inflammatory response. Our study suggests that microwave-pulsed NTAPP may have stronger in vivo antibacterial activity than in vitro activity, and that microwave-pulsed NTAPP may have the additional advantage of suppressing gingival inflammatory responses.",
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Antibacterial activity and effect on gingival cells of microwave-pulsed non-thermal atmospheric pressure plasma in artificial saliva. / Seo, Sang Hee; Han, Ihn; Lee, Han Seol; Choi, Jin Joo; Choi, Eun Ha; Kim, Kyoung Nam; Park, Gyungsoon; Kim, Kwangmahn.

In: Scientific reports, Vol. 7, No. 1, 8395, 01.12.2017.

Research output: Contribution to journalArticle

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AU - Han, Ihn

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AU - Kim, Kyoung Nam

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AU - Kim, Kwangmahn

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