Antibacterial effect of antibiotic solution on cellular viability in canine veins

Jong Chul Park, Hak Joon Sung, Dong Hee Lee, Young Hwan Park, Bum Koo Cho, Hwal Suh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Pretreatment of tissue by using antibiotics is a critical step to prevent microbial contamination before venous transplantation. In this study, the optimal time and temperature of antibiotic solution treatment for maintaining cellular viability with antibacterial effect were investigated. The antibiotic-nutrient solutions were composed of cefoxitin, lincomycin, vancomycin, and polymyxin B in RPMI-1640 medium. After various antibiotic solution treatment times (4, 8, and 12 h) and temperatures (4, 25, and 37°C), the viabilities of cells dissociated from veins (jugular vein, femoral vein, superior vena cava, and inferior vena cava) were determined. Double staining by Griffonia simplicifolia agglutins-fluorescein isothiocyanate (GS1-FITC) and propidium iodide was used. To measure the antibacterial effect of the antibiotic solution, canine veins were artificially infected by 3 kinds of bacteria (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae) and were treated by antibiotic solutions as viability test conditions. After the treatment with the antibiotic solution, the tissue was minced, and the homogenized tissue fraction was cultured on standard method agar. The colony that seemed to be resistant to the antibiotic solution was counted. At 37 and 25°C, the viability of whole cells decreased significantly Asymptotic Significance 2-tailed (Asymp.Sig 2-tailed) < 0.05 after 4 h of antibiotic solution treatment, whereas at 4°C it began to reduce significantly after 8 h of treatment. By antibiotic solution treatment at all 3 temperatures for 4 h, no significant difference in viability of the endothelial cells and whole cells was observed. To maintain the donor vein's cellular viability until transplantation, antibiotic solution treatment for 4 h at 4°C is assumed to be appropriate.

Original languageEnglish
Pages (from-to)490-494
Number of pages5
JournalArtificial Organs
Volume25
Issue number6
DOIs
Publication statusPublished - 2001 Sep 29

Fingerprint

Antibiotics
Canidae
Veins
Anti-Bacterial Agents
Tissue
Temperature
Cell Survival
Griffonia
Transplantation
Cells
Cefoxitin
Polymyxin B
Superior Vena Cava
Femoral Vein
Propidium
Fluorescein-5-isothiocyanate
Jugular Veins
Endothelial cells
Klebsiella pneumoniae
Inferior Vena Cava

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Park, Jong Chul ; Sung, Hak Joon ; Lee, Dong Hee ; Park, Young Hwan ; Cho, Bum Koo ; Suh, Hwal. / Antibacterial effect of antibiotic solution on cellular viability in canine veins. In: Artificial Organs. 2001 ; Vol. 25, No. 6. pp. 490-494.
@article{13010a81b4704a7098f679ed77f39c8e,
title = "Antibacterial effect of antibiotic solution on cellular viability in canine veins",
abstract = "Pretreatment of tissue by using antibiotics is a critical step to prevent microbial contamination before venous transplantation. In this study, the optimal time and temperature of antibiotic solution treatment for maintaining cellular viability with antibacterial effect were investigated. The antibiotic-nutrient solutions were composed of cefoxitin, lincomycin, vancomycin, and polymyxin B in RPMI-1640 medium. After various antibiotic solution treatment times (4, 8, and 12 h) and temperatures (4, 25, and 37°C), the viabilities of cells dissociated from veins (jugular vein, femoral vein, superior vena cava, and inferior vena cava) were determined. Double staining by Griffonia simplicifolia agglutins-fluorescein isothiocyanate (GS1-FITC) and propidium iodide was used. To measure the antibacterial effect of the antibiotic solution, canine veins were artificially infected by 3 kinds of bacteria (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae) and were treated by antibiotic solutions as viability test conditions. After the treatment with the antibiotic solution, the tissue was minced, and the homogenized tissue fraction was cultured on standard method agar. The colony that seemed to be resistant to the antibiotic solution was counted. At 37 and 25°C, the viability of whole cells decreased significantly Asymptotic Significance 2-tailed (Asymp.Sig 2-tailed) < 0.05 after 4 h of antibiotic solution treatment, whereas at 4°C it began to reduce significantly after 8 h of treatment. By antibiotic solution treatment at all 3 temperatures for 4 h, no significant difference in viability of the endothelial cells and whole cells was observed. To maintain the donor vein's cellular viability until transplantation, antibiotic solution treatment for 4 h at 4°C is assumed to be appropriate.",
author = "Park, {Jong Chul} and Sung, {Hak Joon} and Lee, {Dong Hee} and Park, {Young Hwan} and Cho, {Bum Koo} and Hwal Suh",
year = "2001",
month = "9",
day = "29",
doi = "10.1046/j.1525-1594.2001.06706-2.x",
language = "English",
volume = "25",
pages = "490--494",
journal = "Artificial Organs",
issn = "0160-564X",
publisher = "Wiley-Blackwell",
number = "6",

}

Antibacterial effect of antibiotic solution on cellular viability in canine veins. / Park, Jong Chul; Sung, Hak Joon; Lee, Dong Hee; Park, Young Hwan; Cho, Bum Koo; Suh, Hwal.

In: Artificial Organs, Vol. 25, No. 6, 29.09.2001, p. 490-494.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antibacterial effect of antibiotic solution on cellular viability in canine veins

AU - Park, Jong Chul

AU - Sung, Hak Joon

AU - Lee, Dong Hee

AU - Park, Young Hwan

AU - Cho, Bum Koo

AU - Suh, Hwal

PY - 2001/9/29

Y1 - 2001/9/29

N2 - Pretreatment of tissue by using antibiotics is a critical step to prevent microbial contamination before venous transplantation. In this study, the optimal time and temperature of antibiotic solution treatment for maintaining cellular viability with antibacterial effect were investigated. The antibiotic-nutrient solutions were composed of cefoxitin, lincomycin, vancomycin, and polymyxin B in RPMI-1640 medium. After various antibiotic solution treatment times (4, 8, and 12 h) and temperatures (4, 25, and 37°C), the viabilities of cells dissociated from veins (jugular vein, femoral vein, superior vena cava, and inferior vena cava) were determined. Double staining by Griffonia simplicifolia agglutins-fluorescein isothiocyanate (GS1-FITC) and propidium iodide was used. To measure the antibacterial effect of the antibiotic solution, canine veins were artificially infected by 3 kinds of bacteria (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae) and were treated by antibiotic solutions as viability test conditions. After the treatment with the antibiotic solution, the tissue was minced, and the homogenized tissue fraction was cultured on standard method agar. The colony that seemed to be resistant to the antibiotic solution was counted. At 37 and 25°C, the viability of whole cells decreased significantly Asymptotic Significance 2-tailed (Asymp.Sig 2-tailed) < 0.05 after 4 h of antibiotic solution treatment, whereas at 4°C it began to reduce significantly after 8 h of treatment. By antibiotic solution treatment at all 3 temperatures for 4 h, no significant difference in viability of the endothelial cells and whole cells was observed. To maintain the donor vein's cellular viability until transplantation, antibiotic solution treatment for 4 h at 4°C is assumed to be appropriate.

AB - Pretreatment of tissue by using antibiotics is a critical step to prevent microbial contamination before venous transplantation. In this study, the optimal time and temperature of antibiotic solution treatment for maintaining cellular viability with antibacterial effect were investigated. The antibiotic-nutrient solutions were composed of cefoxitin, lincomycin, vancomycin, and polymyxin B in RPMI-1640 medium. After various antibiotic solution treatment times (4, 8, and 12 h) and temperatures (4, 25, and 37°C), the viabilities of cells dissociated from veins (jugular vein, femoral vein, superior vena cava, and inferior vena cava) were determined. Double staining by Griffonia simplicifolia agglutins-fluorescein isothiocyanate (GS1-FITC) and propidium iodide was used. To measure the antibacterial effect of the antibiotic solution, canine veins were artificially infected by 3 kinds of bacteria (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae) and were treated by antibiotic solutions as viability test conditions. After the treatment with the antibiotic solution, the tissue was minced, and the homogenized tissue fraction was cultured on standard method agar. The colony that seemed to be resistant to the antibiotic solution was counted. At 37 and 25°C, the viability of whole cells decreased significantly Asymptotic Significance 2-tailed (Asymp.Sig 2-tailed) < 0.05 after 4 h of antibiotic solution treatment, whereas at 4°C it began to reduce significantly after 8 h of treatment. By antibiotic solution treatment at all 3 temperatures for 4 h, no significant difference in viability of the endothelial cells and whole cells was observed. To maintain the donor vein's cellular viability until transplantation, antibiotic solution treatment for 4 h at 4°C is assumed to be appropriate.

UR - http://www.scopus.com/inward/record.url?scp=0034835035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034835035&partnerID=8YFLogxK

U2 - 10.1046/j.1525-1594.2001.06706-2.x

DO - 10.1046/j.1525-1594.2001.06706-2.x

M3 - Article

C2 - 11453881

AN - SCOPUS:0034835035

VL - 25

SP - 490

EP - 494

JO - Artificial Organs

JF - Artificial Organs

SN - 0160-564X

IS - 6

ER -