Antibacterial photodynamic therapy with curcumin and Curcuma xanthorrhiza extract against Streptococcus mutans

Hyung Jung Lee, Si Mook Kang, Seung Hwa Jeong, Ki Ho Chung, Baekil Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background Bacteria are becoming increasingly resistant to conventional antibacterial chemotherapy. This has prompted the application of antibacterial photodynamic therapy (aPDT) in bacteria-related diseases due to its excellent biocide effects. However, few studies have attempted to develop a novel photosensitizer based on natural components. The aim of the present study was to compare the aPDT effects of curcumin and Curcuma xanthorrhiza extract (CXE) against Streptococcus mutans. Methods A planktonic suspension containing an S. mutans strain was treated in three separate groups: aPDT with curcumin, CXE, and a mixture of curcumin and CXE (ratio= 1:1) at concentrations of 0, 10, 102, 103, and 104 ng/ml. Light irradiation with a center wavelength of 405 nm was applied using an LED (power density of 84.5 mW for 300 s at an energy density of 25.3 J/cm2). The phototoxicity of photosensitizers against S. mutans was investigated using a colony-forming-unit assay. Percentage logarithmic reductions [log10(CFU/ml) values] were analyzed using one-way ANOVA followed by the Tukey test (p < 0.05) and Student's independent t-test. Results The viability of S. mutans in the presence of curcumin, CXE, and a mixture of these two components was substantially reduced during irradiation with 405 nm light. The phototoxicity of the photosensitizer varied with its solubility and concentration. Conclusion These preliminary in vitro findings imply that combining curcumin and CXE with a 405 nm LED may be a novel method of applying aPDT. This could be advantageous in preventing and treating dental caries using devices that are readily available in clinics.

Original languageEnglish
Pages (from-to)116-119
Number of pages4
JournalPhotodiagnosis and Photodynamic Therapy
Volume20
DOIs
Publication statusPublished - 2017 Dec 1

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Curcuma
Curcumin
Streptococcus mutans
Photochemotherapy
Photosensitizing Agents
Phototoxic Dermatitis
Colony-Forming Units Assay
Bacteria
Light
Disinfectants
Dental Caries
Solubility
Suspensions
Analysis of Variance
Students
Drug Therapy
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Oncology
  • Dermatology
  • Pharmacology (medical)

Cite this

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title = "Antibacterial photodynamic therapy with curcumin and Curcuma xanthorrhiza extract against Streptococcus mutans",
abstract = "Background Bacteria are becoming increasingly resistant to conventional antibacterial chemotherapy. This has prompted the application of antibacterial photodynamic therapy (aPDT) in bacteria-related diseases due to its excellent biocide effects. However, few studies have attempted to develop a novel photosensitizer based on natural components. The aim of the present study was to compare the aPDT effects of curcumin and Curcuma xanthorrhiza extract (CXE) against Streptococcus mutans. Methods A planktonic suspension containing an S. mutans strain was treated in three separate groups: aPDT with curcumin, CXE, and a mixture of curcumin and CXE (ratio= 1:1) at concentrations of 0, 10, 102, 103, and 104 ng/ml. Light irradiation with a center wavelength of 405 nm was applied using an LED (power density of 84.5 mW for 300 s at an energy density of 25.3 J/cm2). The phototoxicity of photosensitizers against S. mutans was investigated using a colony-forming-unit assay. Percentage logarithmic reductions [log10(CFU/ml) values] were analyzed using one-way ANOVA followed by the Tukey test (p < 0.05) and Student's independent t-test. Results The viability of S. mutans in the presence of curcumin, CXE, and a mixture of these two components was substantially reduced during irradiation with 405 nm light. The phototoxicity of the photosensitizer varied with its solubility and concentration. Conclusion These preliminary in vitro findings imply that combining curcumin and CXE with a 405 nm LED may be a novel method of applying aPDT. This could be advantageous in preventing and treating dental caries using devices that are readily available in clinics.",
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Antibacterial photodynamic therapy with curcumin and Curcuma xanthorrhiza extract against Streptococcus mutans. / Lee, Hyung Jung; Kang, Si Mook; Jeong, Seung Hwa; Chung, Ki Ho; Kim, Baekil.

In: Photodiagnosis and Photodynamic Therapy, Vol. 20, 01.12.2017, p. 116-119.

Research output: Contribution to journalArticle

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AU - Chung, Ki Ho

AU - Kim, Baekil

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N2 - Background Bacteria are becoming increasingly resistant to conventional antibacterial chemotherapy. This has prompted the application of antibacterial photodynamic therapy (aPDT) in bacteria-related diseases due to its excellent biocide effects. However, few studies have attempted to develop a novel photosensitizer based on natural components. The aim of the present study was to compare the aPDT effects of curcumin and Curcuma xanthorrhiza extract (CXE) against Streptococcus mutans. Methods A planktonic suspension containing an S. mutans strain was treated in three separate groups: aPDT with curcumin, CXE, and a mixture of curcumin and CXE (ratio= 1:1) at concentrations of 0, 10, 102, 103, and 104 ng/ml. Light irradiation with a center wavelength of 405 nm was applied using an LED (power density of 84.5 mW for 300 s at an energy density of 25.3 J/cm2). The phototoxicity of photosensitizers against S. mutans was investigated using a colony-forming-unit assay. Percentage logarithmic reductions [log10(CFU/ml) values] were analyzed using one-way ANOVA followed by the Tukey test (p < 0.05) and Student's independent t-test. Results The viability of S. mutans in the presence of curcumin, CXE, and a mixture of these two components was substantially reduced during irradiation with 405 nm light. The phototoxicity of the photosensitizer varied with its solubility and concentration. Conclusion These preliminary in vitro findings imply that combining curcumin and CXE with a 405 nm LED may be a novel method of applying aPDT. This could be advantageous in preventing and treating dental caries using devices that are readily available in clinics.

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