Antibacterial photodynamic therapy with curcumin and Curcuma xanthorrhiza extract against Streptococcus mutans

Hyung Jung Lee, Si Mook Kang, Seung Hwa Jeong, Ki Ho Chung, Baek Il Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background Bacteria are becoming increasingly resistant to conventional antibacterial chemotherapy. This has prompted the application of antibacterial photodynamic therapy (aPDT) in bacteria-related diseases due to its excellent biocide effects. However, few studies have attempted to develop a novel photosensitizer based on natural components. The aim of the present study was to compare the aPDT effects of curcumin and Curcuma xanthorrhiza extract (CXE) against Streptococcus mutans. Methods A planktonic suspension containing an S. mutans strain was treated in three separate groups: aPDT with curcumin, CXE, and a mixture of curcumin and CXE (ratio= 1:1) at concentrations of 0, 10, 10 2 , 10 3 , and 10 4 ng/ml. Light irradiation with a center wavelength of 405 nm was applied using an LED (power density of 84.5 mW for 300 s at an energy density of 25.3 J/cm 2 ). The phototoxicity of photosensitizers against S. mutans was investigated using a colony-forming-unit assay. Percentage logarithmic reductions [log 10 (CFU/ml) values] were analyzed using one-way ANOVA followed by the Tukey test (p < 0.05) and Student's independent t-test. Results The viability of S. mutans in the presence of curcumin, CXE, and a mixture of these two components was substantially reduced during irradiation with 405 nm light. The phototoxicity of the photosensitizer varied with its solubility and concentration. Conclusion These preliminary in vitro findings imply that combining curcumin and CXE with a 405 nm LED may be a novel method of applying aPDT. This could be advantageous in preventing and treating dental caries using devices that are readily available in clinics.

Original languageEnglish
Pages (from-to)116-119
Number of pages4
JournalPhotodiagnosis and Photodynamic Therapy
Volume20
DOIs
Publication statusPublished - 2017 Dec

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Oncology
  • Dermatology
  • Pharmacology (medical)

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