Korean Source

Translated title of the contribution: Antibiotic resistance mechanisms of Escherichia coli isolates from urinary specimens

Sungwook Song, Eun Young Lee, Eun Mi Koh, Ho Sung Ha, Ho Joong Jeong, Il Kwon Bae, Seokhoon Jeong

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: This study was designed to characterize urinary isolates of Escherichia coli that produce extended-spectrum β-lactamases (ESBLs) and to determine the prevalence of other antimicrobial resistance genes. Methods: A total of 264 non-duplicate clinical isolates of E. coli were recovered from urine specimens in a tertiary-care hospital in Busan in 2005. Antimicrobial susceptibility was determined by disk diffusion and agar dilution methods, ESBL production was confirmed using the double-disk synergy (DDS) test, and antimicrobial resistance genes were detected by direct sequencing of PCR amplification products. E. coli isolates were classified into four phylogenetic biotypes according to the presence of chuA, yjaA, and TSPE4. Results: DDS testing detected ESBLs in 27 (10.2%) of the 264 isolates. The most common type of ESBL was CTX-M-15 (N=14), followed by CTX-M-3 (N=8) and CTX-M-14 (N=6). All of the ESBLproducing isolates were resistant to ciprofloxacin. PCR experiments detected genes encoding DHA-1 and CMY-10 AmpC β-lactamases in one and two isolates, respectively. Also isolated were 5 isolates harboring 16S rRNA methylases, 2 isolates harboring Qnr, and 19 isolates harboring AAC(6')-Ib-cr. Most ESBL-producing isolates clustered within phylogenetic groups B2 (N=14) and D (N=7). Conclusion: CTX-M enzymes were the dominant type of ESBLs in urinary isolates of E. coli, and ESBL-producing isolates frequently contained other antimicrobial resistance genes. More than half of the urinary E. coli isolates harboring CTX-M enzymes were within the phylogenetic group B2.

Original languageKorean
Pages (from-to)17-24
Number of pages8
JournalKorean Journal of Laboratory Medicine
Volume29
Issue number1
DOIs
Publication statusPublished - 2009 Jun 22

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Microbial Drug Resistance
Escherichia coli
Anti-Bacterial Agents
Genes
Polymerase Chain Reaction
Gene encoding
Enzymes
Tertiary Healthcare
Ciprofloxacin
Tertiary Care Centers
Dilution
Agar
Amplification
Urine
Testing
Experiments

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Song, S., Lee, E. Y., Koh, E. M., Ha, H. S., Jeong, H. J., Bae, I. K., & Jeong, S. (2009). Korean Source. Korean Journal of Laboratory Medicine, 29(1), 17-24. https://doi.org/10.3343/kjlm.2009.29.1.17
Song, Sungwook ; Lee, Eun Young ; Koh, Eun Mi ; Ha, Ho Sung ; Jeong, Ho Joong ; Bae, Il Kwon ; Jeong, Seokhoon. / Korean Source. In: Korean Journal of Laboratory Medicine. 2009 ; Vol. 29, No. 1. pp. 17-24.
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abstract = "Background: This study was designed to characterize urinary isolates of Escherichia coli that produce extended-spectrum β-lactamases (ESBLs) and to determine the prevalence of other antimicrobial resistance genes. Methods: A total of 264 non-duplicate clinical isolates of E. coli were recovered from urine specimens in a tertiary-care hospital in Busan in 2005. Antimicrobial susceptibility was determined by disk diffusion and agar dilution methods, ESBL production was confirmed using the double-disk synergy (DDS) test, and antimicrobial resistance genes were detected by direct sequencing of PCR amplification products. E. coli isolates were classified into four phylogenetic biotypes according to the presence of chuA, yjaA, and TSPE4. Results: DDS testing detected ESBLs in 27 (10.2{\%}) of the 264 isolates. The most common type of ESBL was CTX-M-15 (N=14), followed by CTX-M-3 (N=8) and CTX-M-14 (N=6). All of the ESBLproducing isolates were resistant to ciprofloxacin. PCR experiments detected genes encoding DHA-1 and CMY-10 AmpC β-lactamases in one and two isolates, respectively. Also isolated were 5 isolates harboring 16S rRNA methylases, 2 isolates harboring Qnr, and 19 isolates harboring AAC(6')-Ib-cr. Most ESBL-producing isolates clustered within phylogenetic groups B2 (N=14) and D (N=7). Conclusion: CTX-M enzymes were the dominant type of ESBLs in urinary isolates of E. coli, and ESBL-producing isolates frequently contained other antimicrobial resistance genes. More than half of the urinary E. coli isolates harboring CTX-M enzymes were within the phylogenetic group B2.",
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Song, S, Lee, EY, Koh, EM, Ha, HS, Jeong, HJ, Bae, IK & Jeong, S 2009, 'Korean Source', Korean Journal of Laboratory Medicine, vol. 29, no. 1, pp. 17-24. https://doi.org/10.3343/kjlm.2009.29.1.17

Korean Source. / Song, Sungwook; Lee, Eun Young; Koh, Eun Mi; Ha, Ho Sung; Jeong, Ho Joong; Bae, Il Kwon; Jeong, Seokhoon.

In: Korean Journal of Laboratory Medicine, Vol. 29, No. 1, 22.06.2009, p. 17-24.

Research output: Contribution to journalArticle

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AU - Song, Sungwook

AU - Lee, Eun Young

AU - Koh, Eun Mi

AU - Ha, Ho Sung

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AU - Jeong, Seokhoon

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Y1 - 2009/6/22

N2 - Background: This study was designed to characterize urinary isolates of Escherichia coli that produce extended-spectrum β-lactamases (ESBLs) and to determine the prevalence of other antimicrobial resistance genes. Methods: A total of 264 non-duplicate clinical isolates of E. coli were recovered from urine specimens in a tertiary-care hospital in Busan in 2005. Antimicrobial susceptibility was determined by disk diffusion and agar dilution methods, ESBL production was confirmed using the double-disk synergy (DDS) test, and antimicrobial resistance genes were detected by direct sequencing of PCR amplification products. E. coli isolates were classified into four phylogenetic biotypes according to the presence of chuA, yjaA, and TSPE4. Results: DDS testing detected ESBLs in 27 (10.2%) of the 264 isolates. The most common type of ESBL was CTX-M-15 (N=14), followed by CTX-M-3 (N=8) and CTX-M-14 (N=6). All of the ESBLproducing isolates were resistant to ciprofloxacin. PCR experiments detected genes encoding DHA-1 and CMY-10 AmpC β-lactamases in one and two isolates, respectively. Also isolated were 5 isolates harboring 16S rRNA methylases, 2 isolates harboring Qnr, and 19 isolates harboring AAC(6')-Ib-cr. Most ESBL-producing isolates clustered within phylogenetic groups B2 (N=14) and D (N=7). Conclusion: CTX-M enzymes were the dominant type of ESBLs in urinary isolates of E. coli, and ESBL-producing isolates frequently contained other antimicrobial resistance genes. More than half of the urinary E. coli isolates harboring CTX-M enzymes were within the phylogenetic group B2.

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Song S, Lee EY, Koh EM, Ha HS, Jeong HJ, Bae IK et al. Korean Source. Korean Journal of Laboratory Medicine. 2009 Jun 22;29(1):17-24. https://doi.org/10.3343/kjlm.2009.29.1.17