Antibody-based surface plasmon resonance detection of intact viral pathogen

Hyoungwon Baac, József P. Hajós, Jennifer Lee, Donghyun Kim, Sung June Kim, Michael L. Shuler

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus. Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors.

Original languageEnglish
Pages (from-to)815-819
Number of pages5
JournalBiotechnology and Bioengineering
Volume94
Issue number4
DOIs
Publication statusPublished - 2006 Jul 5

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Antibody-based surface plasmon resonance detection of intact viral pathogen'. Together they form a unique fingerprint.

  • Cite this