Antibody-secreting cells with a phenotype of Ki-67low, CD138high, CD31high, and CD38high secrete nonspecific IgM during primary hepatitis a virus infection

Seokchan Hong, Hyun Woong Lee, Dong Yeop Chang, Sooseong You, Jihye Kim, Jun Yong Park, Sang Hoon Ahn, Dongeun Yong, Kwang Hyub Han, Ook Joon Yoo, Eui Cheol Shin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Although studies investigating the nature of Ab-secreting cells (ASCs) during acute infection with influenza or dengue virus found that the ASC response was dominated by virus-specific IgG secretion, the Ag specificity and phenotype of ASCs during primary acute viral infection were not identified. To this end, we investigated the nature of ASCs in direct ex vivo assays from patients with acute hepatitis A caused by primary infection with hepatitis Avirus (HAV).We found that the frequency of CD27highCD38 high ASCs was markedly increased in the peripheral blood during the acute phase of HAV infection. Moreover, substantial numbers of ASCs were non-HAV-specific and dominantly secreted IgM. We detected HAV-specific ASCs by staining with fluorochrome-tagged HAV-VP1 protein. As compared with HAV-specific ASCs, non-HAV-specific ASCs were Ki-67lowCD138 highCD31highCD38high, demonstrating that non-HAV-specific ASCs had a bone marrow plasma cell-like phenotype whereas HAV-specific ASCs had a phenotype typical of circulating plasmablasts. These data suggest that non-HAV-specific ASCs might be mobilized plasma cells from the bone marrow or the spleen, whereas HAV-specific ASCs were newly generated plasmablasts. In this study, we provide evidence that pre-existing plasma cells are released into the circulation and contribute to Ag-nonspecific secretion of IgM during primary HAV infection.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalJournal of Immunology
Volume191
Issue number1
DOIs
Publication statusPublished - 2013 Jul 1

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Antibody-Producing Cells
Hepatitis Viruses
Virus Diseases
Immunoglobulin M
Phenotype
Hepatitis
Plasma Cells
Infection
Dengue Virus
Hepatitis A
Orthomyxoviridae
Fluorescent Dyes
Bone Marrow Cells

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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title = "Antibody-secreting cells with a phenotype of Ki-67low, CD138high, CD31high, and CD38high secrete nonspecific IgM during primary hepatitis a virus infection",
abstract = "Although studies investigating the nature of Ab-secreting cells (ASCs) during acute infection with influenza or dengue virus found that the ASC response was dominated by virus-specific IgG secretion, the Ag specificity and phenotype of ASCs during primary acute viral infection were not identified. To this end, we investigated the nature of ASCs in direct ex vivo assays from patients with acute hepatitis A caused by primary infection with hepatitis Avirus (HAV).We found that the frequency of CD27highCD38 high ASCs was markedly increased in the peripheral blood during the acute phase of HAV infection. Moreover, substantial numbers of ASCs were non-HAV-specific and dominantly secreted IgM. We detected HAV-specific ASCs by staining with fluorochrome-tagged HAV-VP1 protein. As compared with HAV-specific ASCs, non-HAV-specific ASCs were Ki-67lowCD138 highCD31highCD38high, demonstrating that non-HAV-specific ASCs had a bone marrow plasma cell-like phenotype whereas HAV-specific ASCs had a phenotype typical of circulating plasmablasts. These data suggest that non-HAV-specific ASCs might be mobilized plasma cells from the bone marrow or the spleen, whereas HAV-specific ASCs were newly generated plasmablasts. In this study, we provide evidence that pre-existing plasma cells are released into the circulation and contribute to Ag-nonspecific secretion of IgM during primary HAV infection.",
author = "Seokchan Hong and Lee, {Hyun Woong} and Chang, {Dong Yeop} and Sooseong You and Jihye Kim and Park, {Jun Yong} and Ahn, {Sang Hoon} and Dongeun Yong and Han, {Kwang Hyub} and Yoo, {Ook Joon} and Shin, {Eui Cheol}",
year = "2013",
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doi = "10.4049/jimmunol.1203540",
language = "English",
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pages = "127--134",
journal = "Journal of Immunology",
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Antibody-secreting cells with a phenotype of Ki-67low, CD138high, CD31high, and CD38high secrete nonspecific IgM during primary hepatitis a virus infection. / Hong, Seokchan; Lee, Hyun Woong; Chang, Dong Yeop; You, Sooseong; Kim, Jihye; Park, Jun Yong; Ahn, Sang Hoon; Yong, Dongeun; Han, Kwang Hyub; Yoo, Ook Joon; Shin, Eui Cheol.

In: Journal of Immunology, Vol. 191, No. 1, 01.07.2013, p. 127-134.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antibody-secreting cells with a phenotype of Ki-67low, CD138high, CD31high, and CD38high secrete nonspecific IgM during primary hepatitis a virus infection

AU - Hong, Seokchan

AU - Lee, Hyun Woong

AU - Chang, Dong Yeop

AU - You, Sooseong

AU - Kim, Jihye

AU - Park, Jun Yong

AU - Ahn, Sang Hoon

AU - Yong, Dongeun

AU - Han, Kwang Hyub

AU - Yoo, Ook Joon

AU - Shin, Eui Cheol

PY - 2013/7/1

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N2 - Although studies investigating the nature of Ab-secreting cells (ASCs) during acute infection with influenza or dengue virus found that the ASC response was dominated by virus-specific IgG secretion, the Ag specificity and phenotype of ASCs during primary acute viral infection were not identified. To this end, we investigated the nature of ASCs in direct ex vivo assays from patients with acute hepatitis A caused by primary infection with hepatitis Avirus (HAV).We found that the frequency of CD27highCD38 high ASCs was markedly increased in the peripheral blood during the acute phase of HAV infection. Moreover, substantial numbers of ASCs were non-HAV-specific and dominantly secreted IgM. We detected HAV-specific ASCs by staining with fluorochrome-tagged HAV-VP1 protein. As compared with HAV-specific ASCs, non-HAV-specific ASCs were Ki-67lowCD138 highCD31highCD38high, demonstrating that non-HAV-specific ASCs had a bone marrow plasma cell-like phenotype whereas HAV-specific ASCs had a phenotype typical of circulating plasmablasts. These data suggest that non-HAV-specific ASCs might be mobilized plasma cells from the bone marrow or the spleen, whereas HAV-specific ASCs were newly generated plasmablasts. In this study, we provide evidence that pre-existing plasma cells are released into the circulation and contribute to Ag-nonspecific secretion of IgM during primary HAV infection.

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