Abstract
Introduction The aim of this study was to assess the antifungal efficacy of a synthetic human beta-defensin-3-C15 peptide (HBD3-C15) in Candida albicans–infected human root dentin. Methods Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with C. albicans for 3 weeks. They were randomly divided into 4 groups (n = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically. Results All medicaments showed significantly lower CFU than saline (P <.05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (P >.05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (P >.05), and both medicaments had significantly lower CFU than CH at both depths (P <.05). Conclusions In this ex vivo model of C. albicans–infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.
| Original language | English |
|---|---|
| Pages (from-to) | 1857-1861 |
| Number of pages | 5 |
| Journal | Journal of Endodontics |
| Volume | 43 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2017 Nov 1 |
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All Science Journal Classification (ASJC) codes
- Dentistry(all)
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Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on Candida albicans–infected Root Dentin. / Yoo, Yeon Jee; Kwon, Ikyung; Oh, So Ram; Perinpanayagam, Hiran; Lim, Sang Min; Ahn, Ki Bum; Lee, Yoon; Han, Seung Hyun; Chang, Seok Woo; Baek, Seung Ho; Zhu, Qiang; Kum, Kee Yeon.
In: Journal of Endodontics, Vol. 43, No. 11, 01.11.2017, p. 1857-1861.Research output: Contribution to journal › Article
TY - JOUR
T1 - Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on Candida albicans–infected Root Dentin
AU - Yoo, Yeon Jee
AU - Kwon, Ikyung
AU - Oh, So Ram
AU - Perinpanayagam, Hiran
AU - Lim, Sang Min
AU - Ahn, Ki Bum
AU - Lee, Yoon
AU - Han, Seung Hyun
AU - Chang, Seok Woo
AU - Baek, Seung Ho
AU - Zhu, Qiang
AU - Kum, Kee Yeon
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Introduction The aim of this study was to assess the antifungal efficacy of a synthetic human beta-defensin-3-C15 peptide (HBD3-C15) in Candida albicans–infected human root dentin. Methods Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with C. albicans for 3 weeks. They were randomly divided into 4 groups (n = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically. Results All medicaments showed significantly lower CFU than saline (P <.05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (P >.05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (P >.05), and both medicaments had significantly lower CFU than CH at both depths (P <.05). Conclusions In this ex vivo model of C. albicans–infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.
AB - Introduction The aim of this study was to assess the antifungal efficacy of a synthetic human beta-defensin-3-C15 peptide (HBD3-C15) in Candida albicans–infected human root dentin. Methods Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with C. albicans for 3 weeks. They were randomly divided into 4 groups (n = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically. Results All medicaments showed significantly lower CFU than saline (P <.05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (P >.05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (P >.05), and both medicaments had significantly lower CFU than CH at both depths (P <.05). Conclusions In this ex vivo model of C. albicans–infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.
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U2 - 10.1016/j.joen.2017.06.035
DO - 10.1016/j.joen.2017.06.035
M3 - Article
VL - 43
SP - 1857
EP - 1861
JO - Journal of Endodontics
JF - Journal of Endodontics
SN - 0099-2399
IS - 11
ER -