Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on Candida albicans–infected Root Dentin

Yeon Jee Yoo; Ikyung Kwon; So Ram Oh; Hiran Perinpanayagam; Sang Min Lim; Ki Bum Ahn; Yoon Lee; Seung Hyun Han; Seok Woo Chang; Seung Ho Baek; Qiang Zhu; Kee Yeon Kum

doi:10.1016/j.joen.2017.06.035

Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on Candida albicans–infected Root Dentin

Yeon Jee Yoo, Ikyung Kwon, So Ram Oh, Hiran Perinpanayagam, Sang Min Lim, Ki Bum Ahn, Yoon Lee, Seung Hyun Han, Seok Woo Chang, Seung Ho Baek, Qiang Zhu, Kee Yeon Kum

Dentistry

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Introduction The aim of this study was to assess the antifungal efficacy of a synthetic human beta-defensin-3-C15 peptide (HBD3-C15) in Candida albicans–infected human root dentin. Methods Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with C. albicans for 3 weeks. They were randomly divided into 4 groups (n = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically. Results All medicaments showed significantly lower CFU than saline (P <.05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (P >.05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (P >.05), and both medicaments had significantly lower CFU than CH at both depths (P <.05). Conclusions In this ex vivo model of C. albicans–infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.

Original language	English
Pages (from-to)	1857-1861
Number of pages	5
Journal	Journal of endodontics
Volume	43
Issue number	11
DOIs	https://doi.org/10.1016/j.joen.2017.06.035
Publication status	Published - 2017 Nov

Bibliographical note

Funding Information:
Supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), which was funded by the Ministry of Health and Welfare, Republic of Korea (Grant HI14C0469 ).

All Science Journal Classification (ASJC) codes

Dentistry(all)

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Yoo, Yeon Jee ; Kwon, Ikyung ; Oh, So Ram ; Perinpanayagam, Hiran ; Lim, Sang Min ; Ahn, Ki Bum ; Lee, Yoon ; Han, Seung Hyun ; Chang, Seok Woo ; Baek, Seung Ho ; Zhu, Qiang ; Kum, Kee Yeon. / Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on Candida albicans–infected Root Dentin. In: Journal of endodontics. 2017 ; Vol. 43, No. 11. pp. 1857-1861.

@article{25284625bfbe45beaebba220563f7003,

title = "Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on Candida albicans–infected Root Dentin",

abstract = "Introduction The aim of this study was to assess the antifungal efficacy of a synthetic human beta-defensin-3-C15 peptide (HBD3-C15) in Candida albicans–infected human root dentin. Methods Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with C. albicans for 3 weeks. They were randomly divided into 4 groups (n = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically. Results All medicaments showed significantly lower CFU than saline (P <.05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (P >.05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (P >.05), and both medicaments had significantly lower CFU than CH at both depths (P <.05). Conclusions In this ex vivo model of C. albicans–infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.",

author = "Yoo, {Yeon Jee} and Ikyung Kwon and Oh, {So Ram} and Hiran Perinpanayagam and Lim, {Sang Min} and Ahn, {Ki Bum} and Yoon Lee and Han, {Seung Hyun} and Chang, {Seok Woo} and Baek, {Seung Ho} and Qiang Zhu and Kum, {Kee Yeon}",

note = "Funding Information: Supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), which was funded by the Ministry of Health and Welfare, Republic of Korea (Grant HI14C0469 ). ",

year = "2017",

month = nov,

doi = "10.1016/j.joen.2017.06.035",

language = "English",

volume = "43",

pages = "1857--1861",

journal = "Journal of Endodontics",

issn = "0099-2399",

publisher = "Elsevier Inc.",

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Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on Candida albicans–infected Root Dentin. / Yoo, Yeon Jee; Kwon, Ikyung; Oh, So Ram; Perinpanayagam, Hiran; Lim, Sang Min; Ahn, Ki Bum; Lee, Yoon; Han, Seung Hyun; Chang, Seok Woo; Baek, Seung Ho; Zhu, Qiang; Kum, Kee Yeon.

In: Journal of endodontics, Vol. 43, No. 11, 11.2017, p. 1857-1861.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Antifungal Effects of Synthetic Human Beta-defensin-3-C15 Peptide on Candida albicans–infected Root Dentin

AU - Yoo, Yeon Jee

AU - Kwon, Ikyung

AU - Oh, So Ram

AU - Perinpanayagam, Hiran

AU - Lim, Sang Min

AU - Ahn, Ki Bum

AU - Lee, Yoon

AU - Han, Seung Hyun

AU - Chang, Seok Woo

AU - Baek, Seung Ho

AU - Zhu, Qiang

AU - Kum, Kee Yeon

N1 - Funding Information: Supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), which was funded by the Ministry of Health and Welfare, Republic of Korea (Grant HI14C0469 ).

PY - 2017/11

Y1 - 2017/11

N2 - Introduction The aim of this study was to assess the antifungal efficacy of a synthetic human beta-defensin-3-C15 peptide (HBD3-C15) in Candida albicans–infected human root dentin. Methods Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with C. albicans for 3 weeks. They were randomly divided into 4 groups (n = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically. Results All medicaments showed significantly lower CFU than saline (P <.05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (P >.05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (P >.05), and both medicaments had significantly lower CFU than CH at both depths (P <.05). Conclusions In this ex vivo model of C. albicans–infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.

AB - Introduction The aim of this study was to assess the antifungal efficacy of a synthetic human beta-defensin-3-C15 peptide (HBD3-C15) in Candida albicans–infected human root dentin. Methods Standardized root dentin blocks were prepared (6-mm thick, 0.7-mm-wide canal) from single-rooted human permanent premolars and infected with C. albicans for 3 weeks. They were randomly divided into 4 groups (n = 8/group), and their canals were filled with calcium hydroxide (CH), HBD3-C15 peptide, or chlorhexidine digluconate (CHX, 2%) as disinfectants or saline as control. After 1 week of disinfection, dentinal debris were harvested at depths of 200 and 400 μm from the canal lumen, and incubated in Yeast broth for 72 hours at 37°C. Then, colony-forming units (CFU) were measured to assess the antifungal efficacy of each medicament and analyzed statistically. Results All medicaments showed significantly lower CFU than saline (P <.05), and their antifungal efficacies were similar at both 200- and 400-μm tubular depths (P >.05). HBD3-C15 had similar antifungal efficacy to that of CHX at both depths (P >.05), and both medicaments had significantly lower CFU than CH at both depths (P <.05). Conclusions In this ex vivo model of C. albicans–infected human root dentin, the antifungal efficacy of synthetic HBD3-C15 was comparable with CHX.

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