Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus

Jong Soon Kane, Ki Hoon Lee, Mi Hwa Han, Hyunju Lee, Ji Mi Ahn, Sang Bae Han, Gyoonhee Han, Kiho Lee, Song Kyu Park, Hwan Mook Kim

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The present study reports the antiinflammatory activity of a methanol extract isolated from the stem bark of Magnolia kobus (MK). MK potently inhibited lipopolysaccharide (LPS)-induced production of nitric oxide and interleukin-1β (IL-1β) in RAW 264.7 cells, a murine macrophage-like cell line. The secretion of tumor necrosis factor-α (TNF-α) was also suppressed in LPS-stimulated RAW 264.7 cells although the magnitude of inhibition was weaker than that of nitric oxide and IL-1β. The mRNA expressions of inducible nitric oxide synthase (iNOS), IL-1β and TNF-α were also suppressed by MK in LPS-stimulated RAW 264.7 cells. Further study demonstrated that LPS-induced DNA binding of AP-1 and phosphorylation of c-jun TV-terminal kinase (JNK) were inhibited by MK treatment in RAW 264.7 cells, whereas phosphorylation of p38 mitogen-activated protein kinase was unaffected. Moreover, topical application of MK suppressed ear swelling in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation model. Collectively, these results suggest that MK exerts antiinflammatory effects in vitro and in vivo and this might be mediated, at least in part, by blocking AP-1 and JNK activation.

Original languageEnglish
Pages (from-to)883-888
Number of pages6
JournalPhytotherapy Research
Volume22
Issue number7
DOIs
Publication statusPublished - 2008 Jul 1

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Magnolia
Methanol
Anti-Inflammatory Agents
Lipopolysaccharides
Interleukin-1
Transcription Factor AP-1
Nitric Oxide
Tumor Necrosis Factor-alpha
Phosphorylation
MAP Kinase Kinase 4
Tetradecanoylphorbol Acetate
p38 Mitogen-Activated Protein Kinases
Nitric Oxide Synthase Type II
Ear
Macrophages
Inflammation
Cell Line
Messenger RNA
Skin
RAW 264.7 Cells

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Kane, J. S., Lee, K. H., Han, M. H., Lee, H., Ahn, J. M., Han, S. B., ... Kim, H. M. (2008). Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus. Phytotherapy Research, 22(7), 883-888. https://doi.org/10.1002/ptr.2386
Kane, Jong Soon ; Lee, Ki Hoon ; Han, Mi Hwa ; Lee, Hyunju ; Ahn, Ji Mi ; Han, Sang Bae ; Han, Gyoonhee ; Lee, Kiho ; Park, Song Kyu ; Kim, Hwan Mook. / Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus. In: Phytotherapy Research. 2008 ; Vol. 22, No. 7. pp. 883-888.
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Kane, JS, Lee, KH, Han, MH, Lee, H, Ahn, JM, Han, SB, Han, G, Lee, K, Park, SK & Kim, HM 2008, 'Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus', Phytotherapy Research, vol. 22, no. 7, pp. 883-888. https://doi.org/10.1002/ptr.2386

Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus. / Kane, Jong Soon; Lee, Ki Hoon; Han, Mi Hwa; Lee, Hyunju; Ahn, Ji Mi; Han, Sang Bae; Han, Gyoonhee; Lee, Kiho; Park, Song Kyu; Kim, Hwan Mook.

In: Phytotherapy Research, Vol. 22, No. 7, 01.07.2008, p. 883-888.

Research output: Contribution to journalArticle

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