Antimicrobial Efficacy of a Polymeric Chlorhexidine Release Device Using In Vitro Model of Enterococcus faecalis Dentinal Tubule Infection

Yoon Lee; Seung Hyun Han; Sang Hee Hong; Jin Kyung Lee; Hye Ji; Kee Yeon Kum

doi:10.1016/j.joen.2008.04.008

Antimicrobial Efficacy of a Polymeric Chlorhexidine Release Device Using In Vitro Model of Enterococcus faecalis Dentinal Tubule Infection

Yoon Lee, Seung Hyun Han, Sang Hee Hong, Jin Kyung Lee, Hye Ji, Kee Yeon Kum

Dentistry

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

This study aimed to assess the antimicrobial efficacy of a novel polymeric chlorhexidine-controlled release device as an intracanal medicament. One hundred cylindrical dentin blocks prepared from human single-rooted teeth were inoculated with Enterococcus faecalis for 3 weeks. The intracanal medicaments tested were calcium hydroxide, a polymeric chlorhexidine-controlled release device (PCRD), a polymeric controlled release device without chlorhexidine (CHx), 0.2% CHx solution, and sterile saline. Dentin samples (at 200-μm and 400-μm depths) were collected from the medicated canal lumens after 1 week of medication with sterile LightSpeed files and placed in growth medium. Bacterial growth was assessed spectrophotometrically by analysis of optical density (OD) after 24 hours of incubation. The OD values at both depths were significantly lower in the PCRD group than in the other experimental groups (P < .001). These results indicate that a PCRD can be an effective intracanal medicament against E. faecalis.

Original language	English
Pages (from-to)	855-858
Number of pages	4
Journal	Journal of endodontics
Volume	34
Issue number	7
DOIs	https://doi.org/10.1016/j.joen.2008.04.008
Publication status	Published - 2008 Jul

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund, KRF-2007-314-E00186) and grant 03-2007-0090 from the Seoul National University Dental Hospital Research Fund.

All Science Journal Classification (ASJC) codes

Dentistry(all)

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10.1016/j.joen.2008.04.008

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title = "Antimicrobial Efficacy of a Polymeric Chlorhexidine Release Device Using In Vitro Model of Enterococcus faecalis Dentinal Tubule Infection",

abstract = "This study aimed to assess the antimicrobial efficacy of a novel polymeric chlorhexidine-controlled release device as an intracanal medicament. One hundred cylindrical dentin blocks prepared from human single-rooted teeth were inoculated with Enterococcus faecalis for 3 weeks. The intracanal medicaments tested were calcium hydroxide, a polymeric chlorhexidine-controlled release device (PCRD), a polymeric controlled release device without chlorhexidine (CHx), 0.2% CHx solution, and sterile saline. Dentin samples (at 200-μm and 400-μm depths) were collected from the medicated canal lumens after 1 week of medication with sterile LightSpeed files and placed in growth medium. Bacterial growth was assessed spectrophotometrically by analysis of optical density (OD) after 24 hours of incubation. The OD values at both depths were significantly lower in the PCRD group than in the other experimental groups (P < .001). These results indicate that a PCRD can be an effective intracanal medicament against E. faecalis.",

author = "Yoon Lee and Han, {Seung Hyun} and Hong, {Sang Hee} and Lee, {Jin Kyung} and Hye Ji and Kum, {Kee Yeon}",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund, KRF-2007-314-E00186) and grant 03-2007-0090 from the Seoul National University Dental Hospital Research Fund.",

year = "2008",

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doi = "10.1016/j.joen.2008.04.008",

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AU - Lee, Jin Kyung

AU - Ji, Hye

AU - Kum, Kee Yeon

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund, KRF-2007-314-E00186) and grant 03-2007-0090 from the Seoul National University Dental Hospital Research Fund.

PY - 2008/7

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N2 - This study aimed to assess the antimicrobial efficacy of a novel polymeric chlorhexidine-controlled release device as an intracanal medicament. One hundred cylindrical dentin blocks prepared from human single-rooted teeth were inoculated with Enterococcus faecalis for 3 weeks. The intracanal medicaments tested were calcium hydroxide, a polymeric chlorhexidine-controlled release device (PCRD), a polymeric controlled release device without chlorhexidine (CHx), 0.2% CHx solution, and sterile saline. Dentin samples (at 200-μm and 400-μm depths) were collected from the medicated canal lumens after 1 week of medication with sterile LightSpeed files and placed in growth medium. Bacterial growth was assessed spectrophotometrically by analysis of optical density (OD) after 24 hours of incubation. The OD values at both depths were significantly lower in the PCRD group than in the other experimental groups (P < .001). These results indicate that a PCRD can be an effective intracanal medicament against E. faecalis.

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Antimicrobial Efficacy of a Polymeric Chlorhexidine Release Device Using In Vitro Model of Enterococcus faecalis Dentinal Tubule Infection

Abstract

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