Antimicrobial Efficacy of a Polymeric Chlorhexidine Release Device Using In Vitro Model of Enterococcus faecalis Dentinal Tubule Infection

Yoon Lee, Seung Hyun Han, Sang Hee Hong, Jin Kyung Lee, Hye Ji, Kee Yeon Kum

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

This study aimed to assess the antimicrobial efficacy of a novel polymeric chlorhexidine-controlled release device as an intracanal medicament. One hundred cylindrical dentin blocks prepared from human single-rooted teeth were inoculated with Enterococcus faecalis for 3 weeks. The intracanal medicaments tested were calcium hydroxide, a polymeric chlorhexidine-controlled release device (PCRD), a polymeric controlled release device without chlorhexidine (CHx), 0.2% CHx solution, and sterile saline. Dentin samples (at 200-μm and 400-μm depths) were collected from the medicated canal lumens after 1 week of medication with sterile LightSpeed files and placed in growth medium. Bacterial growth was assessed spectrophotometrically by analysis of optical density (OD) after 24 hours of incubation. The OD values at both depths were significantly lower in the PCRD group than in the other experimental groups (P < .001). These results indicate that a PCRD can be an effective intracanal medicament against E. faecalis.

Original languageEnglish
Pages (from-to)855-858
Number of pages4
JournalJournal of Endodontics
Volume34
Issue number7
DOIs
Publication statusPublished - 2008 Jul 1

Fingerprint

Chlorhexidine
Enterococcus faecalis
Equipment and Supplies
Infection
Dentin
Calcium Hydroxide
Growth
Sodium Chloride
In Vitro Techniques
Tooth

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Lee, Yoon ; Han, Seung Hyun ; Hong, Sang Hee ; Lee, Jin Kyung ; Ji, Hye ; Kum, Kee Yeon. / Antimicrobial Efficacy of a Polymeric Chlorhexidine Release Device Using In Vitro Model of Enterococcus faecalis Dentinal Tubule Infection. In: Journal of Endodontics. 2008 ; Vol. 34, No. 7. pp. 855-858.
@article{9609a8447cd84de9ba6877d089e5b76a,
title = "Antimicrobial Efficacy of a Polymeric Chlorhexidine Release Device Using In Vitro Model of Enterococcus faecalis Dentinal Tubule Infection",
abstract = "This study aimed to assess the antimicrobial efficacy of a novel polymeric chlorhexidine-controlled release device as an intracanal medicament. One hundred cylindrical dentin blocks prepared from human single-rooted teeth were inoculated with Enterococcus faecalis for 3 weeks. The intracanal medicaments tested were calcium hydroxide, a polymeric chlorhexidine-controlled release device (PCRD), a polymeric controlled release device without chlorhexidine (CHx), 0.2{\%} CHx solution, and sterile saline. Dentin samples (at 200-μm and 400-μm depths) were collected from the medicated canal lumens after 1 week of medication with sterile LightSpeed files and placed in growth medium. Bacterial growth was assessed spectrophotometrically by analysis of optical density (OD) after 24 hours of incubation. The OD values at both depths were significantly lower in the PCRD group than in the other experimental groups (P < .001). These results indicate that a PCRD can be an effective intracanal medicament against E. faecalis.",
author = "Yoon Lee and Han, {Seung Hyun} and Hong, {Sang Hee} and Lee, {Jin Kyung} and Hye Ji and Kum, {Kee Yeon}",
year = "2008",
month = "7",
day = "1",
doi = "10.1016/j.joen.2008.04.008",
language = "English",
volume = "34",
pages = "855--858",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier Inc.",
number = "7",

}

Antimicrobial Efficacy of a Polymeric Chlorhexidine Release Device Using In Vitro Model of Enterococcus faecalis Dentinal Tubule Infection. / Lee, Yoon; Han, Seung Hyun; Hong, Sang Hee; Lee, Jin Kyung; Ji, Hye; Kum, Kee Yeon.

In: Journal of Endodontics, Vol. 34, No. 7, 01.07.2008, p. 855-858.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antimicrobial Efficacy of a Polymeric Chlorhexidine Release Device Using In Vitro Model of Enterococcus faecalis Dentinal Tubule Infection

AU - Lee, Yoon

AU - Han, Seung Hyun

AU - Hong, Sang Hee

AU - Lee, Jin Kyung

AU - Ji, Hye

AU - Kum, Kee Yeon

PY - 2008/7/1

Y1 - 2008/7/1

N2 - This study aimed to assess the antimicrobial efficacy of a novel polymeric chlorhexidine-controlled release device as an intracanal medicament. One hundred cylindrical dentin blocks prepared from human single-rooted teeth were inoculated with Enterococcus faecalis for 3 weeks. The intracanal medicaments tested were calcium hydroxide, a polymeric chlorhexidine-controlled release device (PCRD), a polymeric controlled release device without chlorhexidine (CHx), 0.2% CHx solution, and sterile saline. Dentin samples (at 200-μm and 400-μm depths) were collected from the medicated canal lumens after 1 week of medication with sterile LightSpeed files and placed in growth medium. Bacterial growth was assessed spectrophotometrically by analysis of optical density (OD) after 24 hours of incubation. The OD values at both depths were significantly lower in the PCRD group than in the other experimental groups (P < .001). These results indicate that a PCRD can be an effective intracanal medicament against E. faecalis.

AB - This study aimed to assess the antimicrobial efficacy of a novel polymeric chlorhexidine-controlled release device as an intracanal medicament. One hundred cylindrical dentin blocks prepared from human single-rooted teeth were inoculated with Enterococcus faecalis for 3 weeks. The intracanal medicaments tested were calcium hydroxide, a polymeric chlorhexidine-controlled release device (PCRD), a polymeric controlled release device without chlorhexidine (CHx), 0.2% CHx solution, and sterile saline. Dentin samples (at 200-μm and 400-μm depths) were collected from the medicated canal lumens after 1 week of medication with sterile LightSpeed files and placed in growth medium. Bacterial growth was assessed spectrophotometrically by analysis of optical density (OD) after 24 hours of incubation. The OD values at both depths were significantly lower in the PCRD group than in the other experimental groups (P < .001). These results indicate that a PCRD can be an effective intracanal medicament against E. faecalis.

UR - http://www.scopus.com/inward/record.url?scp=45249106172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45249106172&partnerID=8YFLogxK

U2 - 10.1016/j.joen.2008.04.008

DO - 10.1016/j.joen.2008.04.008

M3 - Article

VL - 34

SP - 855

EP - 858

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 7

ER -