Antimicrobial medical sutures with caffeic acid phenethyl ester and their in vitro/in vivo biological assessment

Hye Sun Lee, Soo Youn Lee, So Hyun Park, Jin Hyung Lee, Sang Kook Ahn, Young Mook Choi, Doo Jin Choi, Jeong Ho Chang

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8 Citations (Scopus)

Abstract

The work report the in vitro and in vivo assessment of antimicrobial poly(lactic-co-glycolic acid) (PLGA) sutures loaded with noble natural extracts, caffeic acid phenethyl ester (CAPE) from natural propolis. The mechanical characteristics of the medical sutures have been elucidated including tensile strength as a function of biodegradability, and the sustained release profile of CAPE on sutures has been measured as a function of time, concentration and optimization with the aim of maximizing their antimicrobial effect against Staphylococcus aureus and Escherichia DH5α bacteria. Furthermore, in vitro and in vivo antimicrobial assessments-cytotoxicity tests, bacterial reverse mutation (Ames) assays, and micronucleus assays-were achieved to investigate biocompatibility. Real time RT-PCR showed that the antimicrobial effect of CAPE is related to outer membrane damage and reactive oxygen species synthesis in bacteria.

Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalMedChemComm
Volume4
Issue number5
DOIs
Publication statusPublished - 2013 May 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Pharmaceutical Science

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    Lee, H. S., Lee, S. Y., Park, S. H., Lee, J. H., Ahn, S. K., Choi, Y. M., Choi, D. J., & Chang, J. H. (2013). Antimicrobial medical sutures with caffeic acid phenethyl ester and their in vitro/in vivo biological assessment. MedChemComm, 4(5), 777-782. https://doi.org/10.1039/c2md20289a