Antioxidant Activities of an Exopolysaccharide (DeinoPol) Produced by the Extreme Radiation-Resistant Bacterium Deinococcus radiodurans

Shun Mei Lin, Chan Yu Baek, Jong Hyun Jung, Woo Sik Kim, Ha Yeon Song, Ji Hee Lee, Hyun Jung Ji, Yong Zhi, Bo Sun Kang, Yong Sun Bahn, Ho Seong Seo, Sangyong Lim

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Deinococcus radiodurans shows extreme resistance to a range of remarkable environmental stresses. Deinococcal exopolysaccharide (DeinoPol) is a component of the cell wall, but its role in stress resistance has not yet been well-described. In this study, we isolated and characterized DeinoPol from Deinococcus radiodurans R1 strain and investigated its application as an antioxidant agent. Bioinformatic analysis indicated that dra0033, encoding an ExoP-like protein, was involved in DeinoPol biosynthesis, and dra0033 mutation significantly decreased survival rates in response to stresses. Purified DeinoPol consists of different monosaccharides and has a molecular weight of approximately 80 to 100 kDa. DeinoPol also demonstrates highly protective effects on human keratinocytes in response to stress-induced apoptosis by effectively scavenging ROS. Taken together, these findings indicate that DeinoPol is the first reported deinococcal exopolysaccharide that might be used in cosmetics and pharmaceuticals as a safe and attractive radical scavenger.

Original languageEnglish
Article number55
JournalScientific reports
Volume10
Issue number1
DOIs
Publication statusPublished - 2020 Dec 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF-2018K2A206023828 and NRF-2017M2A2A6A02020925 to HSS) and the Nuclear R&D Program of the Ministry of Science and ICT to SL.

Publisher Copyright:
© 2020, The Author(s).

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'Antioxidant Activities of an Exopolysaccharide (DeinoPol) Produced by the Extreme Radiation-Resistant Bacterium Deinococcus radiodurans'. Together they form a unique fingerprint.

Cite this