Abstract
Caffeic acid (CA) is one of the antioxidants found in plants, which protects vascular cells against vascular injuries from oxidative stress. In our previous study, caffeoyl-prolyl-histidine amide (CA-L-Pro-L-His-NH2; CA-PH; a CA derivative) was synthesized, which exhibited a strong antioxidant activity with sufficient stability. In this study, we investigated the role of CA-PH in vascular smooth muscle cells (VSMCs) and confirmed the enhanced antioxidant activity of CA-PH compared with that of CA. In in vitro tube assays, CA-PH showed a higher free-radical-scavenging activity and lipid-peroxidation-inhibition activity than those of CA. In VSMCs, CA-PH significantly reduced hydrogen peroxide-induced ROS generation and increased the expression of heme oxygenase-1. Moreover, CA-PH effectively inhibited the platelet-derived growth factor-induced cellular proliferation of VSMCs, which was confirmed by a decrease in the expression of the proliferating cell nuclear antigen and the phosphorylation of Akt.
Original language | English |
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Pages (from-to) | 2777-2785 |
Number of pages | 9 |
Journal | Amino Acids |
Volume | 46 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2014 Dec |
Bibliographical note
Funding Information:This study was supported by a Grant of the Korea Healthcare technology R&D Project, Ministry for Health & Welfare Affairs, Republic of Korea (HI08C2149).
Publisher Copyright:
© 2014 Springer-Verlag Wien.
All Science Journal Classification (ASJC) codes
- Biochemistry
- Organic Chemistry
- Clinical Biochemistry