Antioxidant properties of neohesperidin dihydrochalcone: Inhibition of hypochlorous acid-induced DNA strand breakage, protein degradation, and cell death

Je Min Choi, Byoung Seok Yoon, Sang Kyou Lee, Jae Kwan Hwang, Ryung Ryang

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Neohesperidin dihydrochalcone (NHDC), a non-nutritive sweetening agent, is simply produced by hydrogenation of neohesperidin. The aim of this study is to evaluate the antioxidant and radical scavenging properties of neohesperidin dihydrochalcone and other structurally related compounds (phloridzin, neohesperidin) toward different reactive radical and oxygen species including ·ABTS+, ·O2-, ·OH, H 2O2, and HOCl in vitro. NHDC showed remarkable radical scavenging activity against stable radical and reactive oxygen species (ROS) in concentration dependent manner. Especially, NHDC was the most potent inhibitor of H2O2 and HOCl. NHDC showed HOCl scavenging activity of 93.5% and H2O2 scavenging property of 73.5% which was more than those of all the tested compounds including ascorbic acid and BHT. Moreover, NHDC could inhibit protein degradation, plasmid DNA strand cleavage and HIT-T15, HUVEC cell death from HOCl attack while mannitol, BHT, and ascorbic acid could not protect them effectively. These results suggest that NHDC is a potent antioxidant, especially it is evaluated as a novel HOCl scavenger. This study implies the possibility of therapeutic effect of NHDC on ROS-related inflammatory diseases.

Original languageEnglish
Pages (from-to)324-330
Number of pages7
JournalBiological and Pharmaceutical Bulletin
Volume30
Issue number2
DOIs
Publication statusPublished - 2007 Feb

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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