Antioxidant properties of neohesperidin dihydrochalcone

Inhibition of hypochlorous acid-induced DNA strand breakage, protein degradation, and cell death

Je Min Choi, Byoung Seok Yoon, Sang Kyou Lee, Jae-Kwan Hwang, Ryung Ryang

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Neohesperidin dihydrochalcone (NHDC), a non-nutritive sweetening agent, is simply produced by hydrogenation of neohesperidin. The aim of this study is to evaluate the antioxidant and radical scavenging properties of neohesperidin dihydrochalcone and other structurally related compounds (phloridzin, neohesperidin) toward different reactive radical and oxygen species including ·ABTS+, ·O2 -, ·OH, H 2O2, and HOCl in vitro. NHDC showed remarkable radical scavenging activity against stable radical and reactive oxygen species (ROS) in concentration dependent manner. Especially, NHDC was the most potent inhibitor of H2O2 and HOCl. NHDC showed HOCl scavenging activity of 93.5% and H2O2 scavenging property of 73.5% which was more than those of all the tested compounds including ascorbic acid and BHT. Moreover, NHDC could inhibit protein degradation, plasmid DNA strand cleavage and HIT-T15, HUVEC cell death from HOCl attack while mannitol, BHT, and ascorbic acid could not protect them effectively. These results suggest that NHDC is a potent antioxidant, especially it is evaluated as a novel HOCl scavenger. This study implies the possibility of therapeutic effect of NHDC on ROS-related inflammatory diseases.

Original languageEnglish
Pages (from-to)324-330
Number of pages7
JournalBiological and Pharmaceutical Bulletin
Volume30
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1

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Hypochlorous Acid
Proteolysis
Cell Death
Antioxidants
DNA
Reactive Oxygen Species
Butylated Hydroxytoluene
Ascorbic Acid
Phlorhizin
Sweetening Agents
DNA Cleavage
Hydrogenation
neohesperidin dihydrochalcone
Human Umbilical Vein Endothelial Cells
Mannitol
Therapeutic Uses
Plasmids

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

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title = "Antioxidant properties of neohesperidin dihydrochalcone: Inhibition of hypochlorous acid-induced DNA strand breakage, protein degradation, and cell death",
abstract = "Neohesperidin dihydrochalcone (NHDC), a non-nutritive sweetening agent, is simply produced by hydrogenation of neohesperidin. The aim of this study is to evaluate the antioxidant and radical scavenging properties of neohesperidin dihydrochalcone and other structurally related compounds (phloridzin, neohesperidin) toward different reactive radical and oxygen species including ·ABTS+, ·O2 -, ·OH, H 2O2, and HOCl in vitro. NHDC showed remarkable radical scavenging activity against stable radical and reactive oxygen species (ROS) in concentration dependent manner. Especially, NHDC was the most potent inhibitor of H2O2 and HOCl. NHDC showed HOCl scavenging activity of 93.5{\%} and H2O2 scavenging property of 73.5{\%} which was more than those of all the tested compounds including ascorbic acid and BHT. Moreover, NHDC could inhibit protein degradation, plasmid DNA strand cleavage and HIT-T15, HUVEC cell death from HOCl attack while mannitol, BHT, and ascorbic acid could not protect them effectively. These results suggest that NHDC is a potent antioxidant, especially it is evaluated as a novel HOCl scavenger. This study implies the possibility of therapeutic effect of NHDC on ROS-related inflammatory diseases.",
author = "Choi, {Je Min} and Yoon, {Byoung Seok} and Lee, {Sang Kyou} and Jae-Kwan Hwang and Ryung Ryang",
year = "2007",
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T2 - Inhibition of hypochlorous acid-induced DNA strand breakage, protein degradation, and cell death

AU - Choi, Je Min

AU - Yoon, Byoung Seok

AU - Lee, Sang Kyou

AU - Hwang, Jae-Kwan

AU - Ryang, Ryung

PY - 2007/2/1

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