Antirepression system associated with the life cycle switch in the temperate podoviridae phage SPC32H

Minsik Kim, Sangryeol Ryu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Prophages switch from lysogenic to lytic mode in response to the host SOS response. The primary factor that governs this switch is a phage repressor, which is typically a host RecA-dependent autocleavable protein. Here, in an effort to reveal the mechanism underlying the phenotypic differences between the Salmonella temperate phages SPC32H and SPC32N, whose genome sequences differ by only two nucleotides, we identified a new class of Podoviridae phage lytic switch antirepressor that is structurally distinct from the previously reported Sipho- and Myoviridae phage antirepressors. The SPC32H repressor (Rep) is not cleaved by the SOS response but instead is inactivated by a small antirepressor (Ant), the expression of which is negatively controlled by host LexA. A single nucleotide mutation in the consensus sequence of the LexA-binding site, which overlaps with the ant promoter, results in constitutive Ant synthesis and consequently induces SPC32N to enter the lytic cycle. Numerous potential Ant homologues were identified in a variety of putative prophages and temperate Podoviridae phages, indicating that antirepressors may be widespread among temperate phages in the order Caudovirales to mediate a prudent prophage induction.

Original languageEnglish
Pages (from-to)11775-11786
Number of pages12
JournalJournal of Virology
Volume87
Issue number21
DOIs
Publication statusPublished - 2013 Oct 25

Fingerprint

Podoviridae
Life Cycle Stages
bacteriophages
Bacteriophages
life cycle (organisms)
Prophages
SOS response (genetics)
Caudovirales
Myoviridae
Nucleotides
Salmonella Phages
Virus Activation
nucleotides
Ants
Consensus Sequence
consensus sequence
Binding Sites
binding sites
Genome
Salmonella

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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Antirepression system associated with the life cycle switch in the temperate podoviridae phage SPC32H. / Kim, Minsik; Ryu, Sangryeol.

In: Journal of Virology, Vol. 87, No. 21, 25.10.2013, p. 11775-11786.

Research output: Contribution to journalArticle

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