Antitumoral effects of recombinant adenovirus YKL-1001, conditionally replicating in α-fetoprotein-producing human liver cancer cells

Jaesung Kim, Boyoung Lee, Jin Seok Kim, Chae Ok Yun, Joo Hang Kim, Yong J. Lee, Chul Hyun Joo, Heuiran Lee

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Selectively replicating recombinant adenovirus has emerged as a novel strategy for the treatment of incurable human cancers. One of the major characteristics of hepatocellular carcinoma is the transcriptional reactivation of α-fetoprotein (AFP). In this study, we evaluated the liver cancer-specific oncolytic potential of E1B 55kDa-deleted recombinant adenovirus (YKL-1001), which retained other E1 genes driven by the AFP promoter. Transient transfection study using luciferase indicated the selective activation of the AFP promoter only in human liver cancer cells secreting AFP (HepG2, Hep3B). YKL-1001 induced both cytopathic effects exclusively in AFP-positive liver cancer cells and the growth inhibition of pre-established Hep3B xenografts. Finally, hematoxylin-eosin staining and the immunohistochemistry to the adenoviral hexon showed a large distributed necrotic area and this implied a wide spread of YKL-1001. Therefore, the present study demonstrated that YKL-1001 holds significant promise as an oncolytic agent for hepatocellular carcinoma.

Original languageEnglish
Pages (from-to)23-32
Number of pages10
JournalCancer Letters
Volume180
Issue number1
DOIs
Publication statusPublished - 2002 Jun 6

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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