Antiviral activity of oroxylin A against coxsackievirus B3 alleviates virus-induced acute pancreatic damage in mice

Bo Eun Kwon; Jae Hyoung Song; Hyuk Hwan Song; Ju Won Kang; Sam Noh Hwang; Ki Jong Rhee; Aeri Shim; Eun Hye Hong; Yeon Jeong Kim; Sang Min Jeon; Sun Young Chang; Dong Eun Kim; Sungchan Cho; Hyun Jeong Ko

doi:10.1371/journal.pone.0155784

Antiviral activity of oroxylin A against coxsackievirus B3 alleviates virus-induced acute pancreatic damage in mice

Bo Eun Kwon, Jae Hyoung Song, Hyuk Hwan Song, Ju Won Kang, Sam Noh Hwang, Ki Jong Rhee, Aeri Shim, Eun Hye Hong, Yeon Jeong Kim, Sang Min Jeon, Sun Young Chang, Dong Eun Kim, Sungchan Cho, Hyun Jeong Ko

Biomedical Laboratory Science

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18 Citations (Scopus)

Abstract

The flavonoids mosloflavone, oroxylin A, and norwogonin, which were purified from Scutellaria baicalensis Georgi, significantly protected Vero cells against Coxsackievirus B3 (CVB3)-induced cell death. To investigate the in vivo antiviral activity of oroxylin A, we intraperitoneally inoculated CVB3 into 4-week-old BALB/c mice. Body weights and blood glucose levels of the mice were decreased after CVB3 infection, and these changes were attenuated by the administration of oroxylin A. Importantly, treatment of mice with oroxylin A reduced viral titers in the pancreas and decreased the serum levels of the inflammatory cytokines including interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α. Additionally, the administration of oroxylin A mitigated the histological pancreatic lesions and apoptotic cell death induced by CVB3 infection and increased the levels of phospho-eIF2α in infected pancreata. The results suggest that oroxylin A may represent a potent antiviral agent against CVB3 infection.

Original language	English
Article number	e0155784
Journal	PloS one
Volume	11
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0155784
Publication status	Published - 2016 May 1

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (NRF-2014R1A2A2A01002576, NRF-2014R1A1A2003820), and was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI15C0450). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2016 Kwon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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10.1371/journal.pone.0155784

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Kwon, B. E., Song, J. H., Song, H. H., Kang, J. W., Hwang, S. N., Rhee, K. J., Shim, A., Hong, E. H., Kim, Y. J., Jeon, S. M., Chang, S. Y., Kim, D. E., Cho, S., & Ko, H. J. (2016). Antiviral activity of oroxylin A against coxsackievirus B3 alleviates virus-induced acute pancreatic damage in mice. PloS one, 11(5), [e0155784]. https://doi.org/10.1371/journal.pone.0155784

Kwon, Bo Eun ; Song, Jae Hyoung ; Song, Hyuk Hwan ; Kang, Ju Won ; Hwang, Sam Noh ; Rhee, Ki Jong ; Shim, Aeri ; Hong, Eun Hye ; Kim, Yeon Jeong ; Jeon, Sang Min ; Chang, Sun Young ; Kim, Dong Eun ; Cho, Sungchan ; Ko, Hyun Jeong. / Antiviral activity of oroxylin A against coxsackievirus B3 alleviates virus-induced acute pancreatic damage in mice. In: PloS one. 2016 ; Vol. 11, No. 5.

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title = "Antiviral activity of oroxylin A against coxsackievirus B3 alleviates virus-induced acute pancreatic damage in mice",

abstract = "The flavonoids mosloflavone, oroxylin A, and norwogonin, which were purified from Scutellaria baicalensis Georgi, significantly protected Vero cells against Coxsackievirus B3 (CVB3)-induced cell death. To investigate the in vivo antiviral activity of oroxylin A, we intraperitoneally inoculated CVB3 into 4-week-old BALB/c mice. Body weights and blood glucose levels of the mice were decreased after CVB3 infection, and these changes were attenuated by the administration of oroxylin A. Importantly, treatment of mice with oroxylin A reduced viral titers in the pancreas and decreased the serum levels of the inflammatory cytokines including interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α. Additionally, the administration of oroxylin A mitigated the histological pancreatic lesions and apoptotic cell death induced by CVB3 infection and increased the levels of phospho-eIF2α in infected pancreata. The results suggest that oroxylin A may represent a potent antiviral agent against CVB3 infection.",

author = "Kwon, {Bo Eun} and Song, {Jae Hyoung} and Song, {Hyuk Hwan} and Kang, {Ju Won} and Hwang, {Sam Noh} and Rhee, {Ki Jong} and Aeri Shim and Hong, {Eun Hye} and Kim, {Yeon Jeong} and Jeon, {Sang Min} and Chang, {Sun Young} and Kim, {Dong Eun} and Sungchan Cho and Ko, {Hyun Jeong}",

note = "Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (NRF-2014R1A2A2A01002576, NRF-2014R1A1A2003820), and was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI15C0450). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2016 Kwon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

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Antiviral activity of oroxylin A against coxsackievirus B3 alleviates virus-induced acute pancreatic damage in mice. / Kwon, Bo Eun; Song, Jae Hyoung; Song, Hyuk Hwan; Kang, Ju Won; Hwang, Sam Noh; Rhee, Ki Jong; Shim, Aeri; Hong, Eun Hye; Kim, Yeon Jeong; Jeon, Sang Min; Chang, Sun Young; Kim, Dong Eun; Cho, Sungchan; Ko, Hyun Jeong.

In: PloS one, Vol. 11, No. 5, e0155784, 01.05.2016.

Research output: Contribution to journal › Article › peer-review

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AU - Kwon, Bo Eun

AU - Song, Jae Hyoung

AU - Song, Hyuk Hwan

AU - Kang, Ju Won

AU - Hwang, Sam Noh

AU - Rhee, Ki Jong

AU - Shim, Aeri

AU - Hong, Eun Hye

AU - Kim, Yeon Jeong

AU - Jeon, Sang Min

AU - Chang, Sun Young

AU - Kim, Dong Eun

AU - Cho, Sungchan

AU - Ko, Hyun Jeong

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PY - 2016/5/1

Y1 - 2016/5/1

N2 - The flavonoids mosloflavone, oroxylin A, and norwogonin, which were purified from Scutellaria baicalensis Georgi, significantly protected Vero cells against Coxsackievirus B3 (CVB3)-induced cell death. To investigate the in vivo antiviral activity of oroxylin A, we intraperitoneally inoculated CVB3 into 4-week-old BALB/c mice. Body weights and blood glucose levels of the mice were decreased after CVB3 infection, and these changes were attenuated by the administration of oroxylin A. Importantly, treatment of mice with oroxylin A reduced viral titers in the pancreas and decreased the serum levels of the inflammatory cytokines including interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α. Additionally, the administration of oroxylin A mitigated the histological pancreatic lesions and apoptotic cell death induced by CVB3 infection and increased the levels of phospho-eIF2α in infected pancreata. The results suggest that oroxylin A may represent a potent antiviral agent against CVB3 infection.

AB - The flavonoids mosloflavone, oroxylin A, and norwogonin, which were purified from Scutellaria baicalensis Georgi, significantly protected Vero cells against Coxsackievirus B3 (CVB3)-induced cell death. To investigate the in vivo antiviral activity of oroxylin A, we intraperitoneally inoculated CVB3 into 4-week-old BALB/c mice. Body weights and blood glucose levels of the mice were decreased after CVB3 infection, and these changes were attenuated by the administration of oroxylin A. Importantly, treatment of mice with oroxylin A reduced viral titers in the pancreas and decreased the serum levels of the inflammatory cytokines including interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α. Additionally, the administration of oroxylin A mitigated the histological pancreatic lesions and apoptotic cell death induced by CVB3 infection and increased the levels of phospho-eIF2α in infected pancreata. The results suggest that oroxylin A may represent a potent antiviral agent against CVB3 infection.

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Antiviral activity of oroxylin A against coxsackievirus B3 alleviates virus-induced acute pancreatic damage in mice

Abstract

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