Antiviral effect of amphotericin B on Japanese encephalitis virus replication

Hun Kim, Seong Jun Kim, Sue Nie Park, Jong-Won Oh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Amphotericin B (AmB), an amphipathic polyene macrolide, is an antifungal drug produced by Streptomyces nodosus. Recently, AmB has been shown to exert antiviral activity against rubella virus and human immunodeficiency virus by different mechanisms. In this study, we evaluated the antiviral effect of AmB against Japanese encephalitis virus (JEV) and investigated which step of the viral life cycle was inhibited by AmB to understand the mechanism of antiviral action of AmB. AmB reduced both plaque size and number in the infected cells in a dose-dependent manner. In addition, a 200-fold reduction of infectious virus titer was observed by treatment of infected cells with 5 μg/ml of AmB. AmB acted at the post virus-infection step, but not during adsorption of virus to host cells. Western blot analysis revealed that the accumulated level of JEV envelope protein dramatically decreased in the infected cells by treatment with 5-10 μg/ml of AmB. Our results indicate that AmB inhibits the replication of JEV at the postinfection step by interfering with viral replication and/or by inhibiting the synthesis of viral proteins.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalJournal of microbiology and biotechnology
Volume14
Issue number1
Publication statusPublished - 2004 Feb 1

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Japanese Encephalitis Virus
Amphotericin B
Antiviral Agents
Rubella virus
Polyenes
Macrolides
Streptomyces
Viral Proteins
Virus Diseases
Life Cycle Stages
Viral Load
Adsorption

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "Amphotericin B (AmB), an amphipathic polyene macrolide, is an antifungal drug produced by Streptomyces nodosus. Recently, AmB has been shown to exert antiviral activity against rubella virus and human immunodeficiency virus by different mechanisms. In this study, we evaluated the antiviral effect of AmB against Japanese encephalitis virus (JEV) and investigated which step of the viral life cycle was inhibited by AmB to understand the mechanism of antiviral action of AmB. AmB reduced both plaque size and number in the infected cells in a dose-dependent manner. In addition, a 200-fold reduction of infectious virus titer was observed by treatment of infected cells with 5 μg/ml of AmB. AmB acted at the post virus-infection step, but not during adsorption of virus to host cells. Western blot analysis revealed that the accumulated level of JEV envelope protein dramatically decreased in the infected cells by treatment with 5-10 μg/ml of AmB. Our results indicate that AmB inhibits the replication of JEV at the postinfection step by interfering with viral replication and/or by inhibiting the synthesis of viral proteins.",
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Antiviral effect of amphotericin B on Japanese encephalitis virus replication. / Kim, Hun; Kim, Seong Jun; Park, Sue Nie; Oh, Jong-Won.

In: Journal of microbiology and biotechnology, Vol. 14, No. 1, 01.02.2004, p. 121-127.

Research output: Contribution to journalArticle

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