Antiviral effect of catechins in green tea on influenza virus

Jae Min Song, Kwang Hee Lee, Baik Lin Seong

Research output: Contribution to journalArticle

376 Citations (Scopus)

Abstract

Polyphenolic compound catechins ((-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG) and (-)-epigallocatechin (EGC)) from green tea were evaluated for their ability to inhibit influenza virus replication in cell culture and for potentially direct virucidal effect. Among the test compounds, the EGCG and ECG were found to be potent inhibitors of influenza virus replication in MDCK cell culture and this effect was observed in all influenza virus subtypes tested, including A/H1N1, A/H3N2 and B virus. The 50% effective inhibition concentration (EC50) of EGCG, ECG, and EGC for influenza A virus were 22-28, 22-40 and 309-318 μM, respectively. EGCG and ECG exhibited hemagglutination inhibition activity, EGCG being more effective. However, the sensitivity in hemagglutination inhibition was widely different among three different subtypes of influenza viruses tested. Quantitative RT-PCR analysis revealed that, at high concentration, EGCG and ECG also suppressed viral RNA synthesis in MDCK cells whereas EGC failed to show similar effect. Similarly, EGCG and ECG inhibited the neuraminidase activity more effectively than the EGC. The results show that the 3-galloyl group of catechin skeleton plays an important role on the observed antiviral activity, whereas the 5′-OH at the trihydroxy benzyl moiety at 2-position plays a minor role. The results, along with the HA type-specific effect, suggest that the antiviral effect of catechins on influenza virus is mediated not only by specific interaction with HA, but altering the physical properties of viral membrane.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalAntiviral Research
Volume68
Issue number2
DOIs
Publication statusPublished - 2005 Nov 1

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Catechin
Tea
Orthomyxoviridae
Antiviral Agents
Madin Darby Canine Kidney Cells
Hemagglutination
Virus Replication
Cell Culture Techniques
H3N2 Subtype Influenza A Virus
Cercopithecine Herpesvirus 1
Influenza A virus
Viral RNA
Neuraminidase
epigallocatechin gallate
Skeleton
epigallocatechin-(4-8,2-O-7)epicatechin
epicatechin gallate
Polymerase Chain Reaction
Membranes
gallocatechol

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Virology

Cite this

Song, Jae Min ; Lee, Kwang Hee ; Seong, Baik Lin. / Antiviral effect of catechins in green tea on influenza virus. In: Antiviral Research. 2005 ; Vol. 68, No. 2. pp. 66-74.
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Antiviral effect of catechins in green tea on influenza virus. / Song, Jae Min; Lee, Kwang Hee; Seong, Baik Lin.

In: Antiviral Research, Vol. 68, No. 2, 01.11.2005, p. 66-74.

Research output: Contribution to journalArticle

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