Antiwrinkle effect of adipose-derived stem cell

Activation of dermal fibroblast by secretory factors

Won Serk Kim, Byung Soon Park, So Hyun Park, Hyung Ki Kim, Jong Hyuk Sung

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

Background: Adipose-derived stem cells (ADSC) have wound-healing and antioxidant effects on human skin via secretion of growth factors and activation of dermal fibroblasts. Objective: Paracrine mechanism reducing ultraviolet-B (UVB)-induced wrinkles by ADSC is investigated in this study. Methods and Results: Wrinkles were induced by an eight-week UVB irradiation, and were significantly improved by the subcutaneous injection of ADSC in hairless mice. In a replica analysis, parameters involving wrinkles were improved with mid-level and high doses of ADSC (1 × 104 and 1 × 105 cells). Dermal thickness and collagen contents in the dermis also were increased in the ADSC-injected groups. To characterize the paracrine mechanism involving the antiwrinkle effect of ADSC, a conditioned medium of ADSC (ADSC-CM) was directly incubated in human dermal fibroblasts (HDF). UVB irradiation reduced the proliferation of HDF, but this was reversed by the pretreatment of ADSC-CM in a dose-dependent manner. In a cell cycle analysis, ADSC-CM decreased the UVB-induced apoptotic cell death, which was demonstrated by the reduced sub-G1 phase of HDF. In addition, the ADSC-CM increased the protein expression of collagen type I and decreased the protein level of matrix mataloproteinase 1 in HDF, which may account for the increased collagen contents in the dermis. Conclusions: Collectively, these results indicate that the ADSC and its secretory factors are effective for UVB-induced wrinkles, and the antiwrinkle effect is mainly mediated by reducing UVB-induced apoptosis and stimulating collagen synthesis of HDF.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalJournal of Dermatological Science
Volume53
Issue number2
DOIs
Publication statusPublished - 2009 Feb 1

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Fibroblasts
Stem cells
Stem Cells
Chemical activation
Skin
Collagen
Dermis
Irradiation
Hairless Mouse
G1 Phase
Cell death
Subcutaneous Injections
Conditioned Culture Medium
Collagen Type I
Wound Healing
Intercellular Signaling Peptides and Proteins
Cell Cycle
Proteins
Cell Death
Antioxidants

All Science Journal Classification (ASJC) codes

  • Dermatology
  • Biochemistry
  • Molecular Biology

Cite this

Kim, Won Serk ; Park, Byung Soon ; Park, So Hyun ; Kim, Hyung Ki ; Sung, Jong Hyuk. / Antiwrinkle effect of adipose-derived stem cell : Activation of dermal fibroblast by secretory factors. In: Journal of Dermatological Science. 2009 ; Vol. 53, No. 2. pp. 96-102.
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abstract = "Background: Adipose-derived stem cells (ADSC) have wound-healing and antioxidant effects on human skin via secretion of growth factors and activation of dermal fibroblasts. Objective: Paracrine mechanism reducing ultraviolet-B (UVB)-induced wrinkles by ADSC is investigated in this study. Methods and Results: Wrinkles were induced by an eight-week UVB irradiation, and were significantly improved by the subcutaneous injection of ADSC in hairless mice. In a replica analysis, parameters involving wrinkles were improved with mid-level and high doses of ADSC (1 × 104 and 1 × 105 cells). Dermal thickness and collagen contents in the dermis also were increased in the ADSC-injected groups. To characterize the paracrine mechanism involving the antiwrinkle effect of ADSC, a conditioned medium of ADSC (ADSC-CM) was directly incubated in human dermal fibroblasts (HDF). UVB irradiation reduced the proliferation of HDF, but this was reversed by the pretreatment of ADSC-CM in a dose-dependent manner. In a cell cycle analysis, ADSC-CM decreased the UVB-induced apoptotic cell death, which was demonstrated by the reduced sub-G1 phase of HDF. In addition, the ADSC-CM increased the protein expression of collagen type I and decreased the protein level of matrix mataloproteinase 1 in HDF, which may account for the increased collagen contents in the dermis. Conclusions: Collectively, these results indicate that the ADSC and its secretory factors are effective for UVB-induced wrinkles, and the antiwrinkle effect is mainly mediated by reducing UVB-induced apoptosis and stimulating collagen synthesis of HDF.",
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Antiwrinkle effect of adipose-derived stem cell : Activation of dermal fibroblast by secretory factors. / Kim, Won Serk; Park, Byung Soon; Park, So Hyun; Kim, Hyung Ki; Sung, Jong Hyuk.

In: Journal of Dermatological Science, Vol. 53, No. 2, 01.02.2009, p. 96-102.

Research output: Contribution to journalArticle

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