Objectives: We investigated the possible effect of apolipoprotein E (apo E) polymorphism on hypertriglyceridemia in type II diabetes mellitus (DM) patients by both apo E genotyping and phenotyping methods. Design and Methods: Eighty Korean type II DM patients were evaluated. Restriction isotyping after DNA amplification was used for apo E genotyping. The isoelectric focusing of neuraminidase-treated sera followed by immunoblotting was used for apo E phenotyping. Results: The concordant rate between apo E genotyping and phenotyping was 96.3%. Apo E genotype frequencies for all 77 concordant cases were as follows: 72.7% for ε3/3; 16.9% for ε3/4; 7.8% for ε2/3; 1.3% for ε2/4, 1.3% for ε4/4; and 0% for ε2/2. There were no significant differences in apo E genotype frequencies between hypertriglyceridemic (n = 42) and normotriglyceridemic (n = 35) groups. Conclusions: Our findings could not support the association between apo E polymorphism and hypertriglyceridemia among type II DM patients.
Bibliographical noteFunding Information:
This work was partially supported by the research fund (No. 1993-48) for professors of Yonsei University College of Medicine. We are grateful to Dr. P. Haydn Pritchard and Dr. Jean-Francois Bowden in St. Paul's Hospital Lipid Clinic, University of British Columbia, Vancouver, Canada for their kind demonstration of apo E phenotyping. We also thank Dr. Kyung Soon Song in the Department of Clinical Pathology, Dr. Kyung-Sup Kim in the Department of Biochemistry and Molecular Biology, and Dr. Hyun Chul Lee in the Department of Internal Medicine for advice, and Jong Shin Chung and Dr. Young Sook Park for technical assistances in the Department of Clinical Pathology, Yonsei University College of Medicine, Seoul, Korea. We also thank Dr. Gustav Schonfeld, Kountz Professor of Medicine, Washington University School of Medicine in St. Louis, MO, USA for critiquing this manuscript.
All Science Journal Classification (ASJC) codes
- Clinical Biochemistry