Apolipoprotein E genotyping and phenotyping in type II diabetes mellitus patients with hypertriglyceridemia

Jeong Ho Kim, Eun Jig Lee, Oh Hun Kwon

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives: We investigated the possible effect of apolipoprotein E (apo E) polymorphism on hypertriglyceridemia in type II diabetes mellitus (DM) patients by both apo E genotyping and phenotyping methods. Design and Methods: Eighty Korean type II DM patients were evaluated. Restriction isotyping after DNA amplification was used for apo E genotyping. The isoelectric focusing of neuraminidase-treated sera followed by immunoblotting was used for apo E phenotyping. Results: The concordant rate between apo E genotyping and phenotyping was 96.3%. Apo E genotype frequencies for all 77 concordant cases were as follows: 72.7% for ε3/3; 16.9% for ε3/4; 7.8% for ε2/3; 1.3% for ε2/4, 1.3% for ε4/4; and 0% for ε2/2. There were no significant differences in apo E genotype frequencies between hypertriglyceridemic (n = 42) and normotriglyceridemic (n = 35) groups. Conclusions: Our findings could not support the association between apo E polymorphism and hypertriglyceridemia among type II DM patients.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalClinical Biochemistry
Volume30
Issue number1
DOIs
Publication statusPublished - 1997 Feb

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Apolipoprotein E genotyping and phenotyping in type II diabetes mellitus patients with hypertriglyceridemia'. Together they form a unique fingerprint.

Cite this