Apoptosis in dilated cardiomyopathy.

B. K. Hong, H. M. Kwon, K. H. Byun, D. Kim, E. Y. Choi, T. S. Kang, S. M. Kang, K. J. Chun, Y. Jang, H. S. Kim, M. Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

OBJECTIVE: Cardiomyopathy, a popular diagnosis that always obscures more than it reveals, nevertheless has several characteristic histological features. These prominently include widespread focal myocardial fibrosis and associated hypertrophy of surviving cardiac myocyte. In fact, focal noninflammatory degeneration (not necrosis) has been demonstrated as a feature of many forms of cardiac hypertrophy. We hypothesized that this loss of myocardial cells in dilated cardiomyopathy (DCMP) may result from cell death by apoptosis. METHODS: Endomyocardial biopsy specimens from the right ventricles of six patients who suffered from DCMP were studied, and myocardial specimens from two persons who died in motor vehicle accidents were used as negative controls. For identification of apoptosis, immunohistochemistry with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling was performed. In addition, apoptosis was confirmed morphologically by confocal laser scanning microscopy with propidium iodide. RESULTS: Apoptosis, that was represented by an apoptotic index ranging from 19.8 to 25.4%, could be extensively seen in myocytes and also rarely in non-myocytes of interstitium and vascular endothelium. Morphologically, there were a lot of nuclei with clumps of condensed chromatin, suggestive of apoptosis. CONCLUSION: The present study demonstrated that myocyte loss in DCMP might be mainly due to the apoptosis of myocytes and interstitial cells, rather than inflammation or cell necrosis.

Original languageEnglish
Pages (from-to)56-64
Number of pages9
JournalThe Korean journal of internal medicine
Volume15
Issue number1
DOIs
Publication statusPublished - 2000 Jan

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Dilated Cardiomyopathy
Apoptosis
Muscle Cells
Necrosis
DNA Nucleotidylexotransferase
Propidium
Vascular Endothelium
Cardiomegaly
Motor Vehicles
Biotin
Cardiomyopathies
Cardiac Myocytes
Confocal Microscopy
Hypertrophy
Chromatin
Heart Ventricles
Accidents
Fibrosis
Cell Death
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Hong, B. K., Kwon, H. M., Byun, K. H., Kim, D., Choi, E. Y., Kang, T. S., ... Kim, M. (2000). Apoptosis in dilated cardiomyopathy. The Korean journal of internal medicine, 15(1), 56-64. https://doi.org/10.3904/kjim.2000.15.1.56
Hong, B. K. ; Kwon, H. M. ; Byun, K. H. ; Kim, D. ; Choi, E. Y. ; Kang, T. S. ; Kang, S. M. ; Chun, K. J. ; Jang, Y. ; Kim, H. S. ; Kim, M. / Apoptosis in dilated cardiomyopathy. In: The Korean journal of internal medicine. 2000 ; Vol. 15, No. 1. pp. 56-64.
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abstract = "OBJECTIVE: Cardiomyopathy, a popular diagnosis that always obscures more than it reveals, nevertheless has several characteristic histological features. These prominently include widespread focal myocardial fibrosis and associated hypertrophy of surviving cardiac myocyte. In fact, focal noninflammatory degeneration (not necrosis) has been demonstrated as a feature of many forms of cardiac hypertrophy. We hypothesized that this loss of myocardial cells in dilated cardiomyopathy (DCMP) may result from cell death by apoptosis. METHODS: Endomyocardial biopsy specimens from the right ventricles of six patients who suffered from DCMP were studied, and myocardial specimens from two persons who died in motor vehicle accidents were used as negative controls. For identification of apoptosis, immunohistochemistry with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling was performed. In addition, apoptosis was confirmed morphologically by confocal laser scanning microscopy with propidium iodide. RESULTS: Apoptosis, that was represented by an apoptotic index ranging from 19.8 to 25.4{\%}, could be extensively seen in myocytes and also rarely in non-myocytes of interstitium and vascular endothelium. Morphologically, there were a lot of nuclei with clumps of condensed chromatin, suggestive of apoptosis. CONCLUSION: The present study demonstrated that myocyte loss in DCMP might be mainly due to the apoptosis of myocytes and interstitial cells, rather than inflammation or cell necrosis.",
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Hong, BK, Kwon, HM, Byun, KH, Kim, D, Choi, EY, Kang, TS, Kang, SM, Chun, KJ, Jang, Y, Kim, HS & Kim, M 2000, 'Apoptosis in dilated cardiomyopathy.', The Korean journal of internal medicine, vol. 15, no. 1, pp. 56-64. https://doi.org/10.3904/kjim.2000.15.1.56

Apoptosis in dilated cardiomyopathy. / Hong, B. K.; Kwon, H. M.; Byun, K. H.; Kim, D.; Choi, E. Y.; Kang, T. S.; Kang, S. M.; Chun, K. J.; Jang, Y.; Kim, H. S.; Kim, M.

In: The Korean journal of internal medicine, Vol. 15, No. 1, 01.2000, p. 56-64.

Research output: Contribution to journalArticle

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T1 - Apoptosis in dilated cardiomyopathy.

AU - Hong, B. K.

AU - Kwon, H. M.

AU - Byun, K. H.

AU - Kim, D.

AU - Choi, E. Y.

AU - Kang, T. S.

AU - Kang, S. M.

AU - Chun, K. J.

AU - Jang, Y.

AU - Kim, H. S.

AU - Kim, M.

PY - 2000/1

Y1 - 2000/1

N2 - OBJECTIVE: Cardiomyopathy, a popular diagnosis that always obscures more than it reveals, nevertheless has several characteristic histological features. These prominently include widespread focal myocardial fibrosis and associated hypertrophy of surviving cardiac myocyte. In fact, focal noninflammatory degeneration (not necrosis) has been demonstrated as a feature of many forms of cardiac hypertrophy. We hypothesized that this loss of myocardial cells in dilated cardiomyopathy (DCMP) may result from cell death by apoptosis. METHODS: Endomyocardial biopsy specimens from the right ventricles of six patients who suffered from DCMP were studied, and myocardial specimens from two persons who died in motor vehicle accidents were used as negative controls. For identification of apoptosis, immunohistochemistry with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling was performed. In addition, apoptosis was confirmed morphologically by confocal laser scanning microscopy with propidium iodide. RESULTS: Apoptosis, that was represented by an apoptotic index ranging from 19.8 to 25.4%, could be extensively seen in myocytes and also rarely in non-myocytes of interstitium and vascular endothelium. Morphologically, there were a lot of nuclei with clumps of condensed chromatin, suggestive of apoptosis. CONCLUSION: The present study demonstrated that myocyte loss in DCMP might be mainly due to the apoptosis of myocytes and interstitial cells, rather than inflammation or cell necrosis.

AB - OBJECTIVE: Cardiomyopathy, a popular diagnosis that always obscures more than it reveals, nevertheless has several characteristic histological features. These prominently include widespread focal myocardial fibrosis and associated hypertrophy of surviving cardiac myocyte. In fact, focal noninflammatory degeneration (not necrosis) has been demonstrated as a feature of many forms of cardiac hypertrophy. We hypothesized that this loss of myocardial cells in dilated cardiomyopathy (DCMP) may result from cell death by apoptosis. METHODS: Endomyocardial biopsy specimens from the right ventricles of six patients who suffered from DCMP were studied, and myocardial specimens from two persons who died in motor vehicle accidents were used as negative controls. For identification of apoptosis, immunohistochemistry with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling was performed. In addition, apoptosis was confirmed morphologically by confocal laser scanning microscopy with propidium iodide. RESULTS: Apoptosis, that was represented by an apoptotic index ranging from 19.8 to 25.4%, could be extensively seen in myocytes and also rarely in non-myocytes of interstitium and vascular endothelium. Morphologically, there were a lot of nuclei with clumps of condensed chromatin, suggestive of apoptosis. CONCLUSION: The present study demonstrated that myocyte loss in DCMP might be mainly due to the apoptosis of myocytes and interstitial cells, rather than inflammation or cell necrosis.

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Hong BK, Kwon HM, Byun KH, Kim D, Choi EY, Kang TS et al. Apoptosis in dilated cardiomyopathy. The Korean journal of internal medicine. 2000 Jan;15(1):56-64. https://doi.org/10.3904/kjim.2000.15.1.56