Apple proteins that interact with DspA/E, a pathogenicity effector of Erwinia amylovora, the fire blight pathogen

Xiangdong Meng, Jean M. Bonasera, Jihyun F. Kim, Riitta M. Nissinen, Steven V. Beer

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The disease-specific (dsp) gene dspA/E of Erwinia amylovora encodes an essential pathogenicity effector of 198 RDa, which is critical to the development of the devastating plant disease fire blight. A yeast two-hybrid assay and in vitro protein pull-down assay demonstrated that DspA/E interacts physically and specifically with four similar putative leucine-rich repeat (LRR) receptor-like serine/threonine kinases (RLK) from apple, an important host of E. amylovora. The genes encoding these four DspA/E-interacting proteins of Malus xdomestica (DIPM1 to 4) are conserved in all genera of hosts of E. amylovora tested. They also are conserved in all cultivars of apple tested that range in susceptibility to fire blight from highly susceptible to highly resistant. The four DIPMs have been characterized, and they are expressed constitutively in host plants. In silico analysis indicated that the DIPMs have similar sequence structure and resemble LRR RLKs from other organisms. Evidence is presented for direct physical interaction between DspA/E and the apple proteins encoded by the four identified clones, which may act as susceptibility factors and be essential to disease development. Knowledge of DIPMs and the interaction with DspA/E thus may facilitate understanding of fire blight development and lead to new approaches to control of disease.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalMolecular Plant-Microbe Interactions
Volume19
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

Fingerprint

Erwinia amylovora
Malus
Virulence
pathogenicity
apples
pathogens
Leucine
Proteins
proteins
Plant Diseases
Two-Hybrid System Techniques
leucine
Protein-Serine-Threonine Kinases
Computer Simulation
Genes
two hybrid system techniques
Clone Cells
plant diseases and disorders
threonine
serine

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cite this

Meng, Xiangdong ; Bonasera, Jean M. ; Kim, Jihyun F. ; Nissinen, Riitta M. ; Beer, Steven V. / Apple proteins that interact with DspA/E, a pathogenicity effector of Erwinia amylovora, the fire blight pathogen. In: Molecular Plant-Microbe Interactions. 2006 ; Vol. 19, No. 1. pp. 53-61.
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Apple proteins that interact with DspA/E, a pathogenicity effector of Erwinia amylovora, the fire blight pathogen. / Meng, Xiangdong; Bonasera, Jean M.; Kim, Jihyun F.; Nissinen, Riitta M.; Beer, Steven V.

In: Molecular Plant-Microbe Interactions, Vol. 19, No. 1, 01.01.2006, p. 53-61.

Research output: Contribution to journalArticle

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