Application of a functionalized parylene film as a linker layer of SPR biosensor

Byoung Jin Jeon, Moo Hwan Kim, Jae Chul Pyun

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The surface plasmon resonance (SPR) biosensors have been used to detect various target analytes by using highly specific antigen-antibody interactions. In this work, a parylene film modified to have primary amine groups was applied as a linker layer of the SPR biosensor, and the primary amine groups were used for the covalent immobilization of proteins to the SPR biosensor. The feasibility of the parylene film as a linker layer was presented by estimating the influence of the parylene film on the SPR measurement parameters, such as the sensitivity and the detection range. Then, a model protein called horseradish peroxidase (HRP) was used to demonstrate the improved immobilization efficiency as well as the sensitivity of the SPR biosensor with the parylene-A film. Additionally, a reconstruction method of the immunoaffinity layer was presented by using oxygen plasma.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume154
Issue number2
DOIs
Publication statusPublished - 2011 Jun 20

Fingerprint

Surface plasmon resonance
bioinstrumentation
surface plasmon resonance
Biosensors
immobilization
Amines
amines
proteins
Proteins
sensitivity
oxygen plasma
antigens
Horseradish Peroxidase
Antigens
antibodies
Antibodies
estimating
parylene
Oxygen
Plasmas

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

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Application of a functionalized parylene film as a linker layer of SPR biosensor. / Jeon, Byoung Jin; Kim, Moo Hwan; Pyun, Jae Chul.

In: Sensors and Actuators, B: Chemical, Vol. 154, No. 2, 20.06.2011, p. 89-95.

Research output: Contribution to journalArticle

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