Application of a functionalized parylene film as a linker layer of SPR biosensor

Byoung Jin Jeon, Moo Hwan Kim, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)


The surface plasmon resonance (SPR) biosensors have been used to detect various target analytes by using highly specific antigen-antibody interactions. In this work, a parylene film modified to have primary amine groups was applied as a linker layer of the SPR biosensor, and the primary amine groups were used for the covalent immobilization of proteins to the SPR biosensor. The feasibility of the parylene film as a linker layer was presented by estimating the influence of the parylene film on the SPR measurement parameters, such as the sensitivity and the detection range. Then, a model protein called horseradish peroxidase (HRP) was used to demonstrate the improved immobilization efficiency as well as the sensitivity of the SPR biosensor with the parylene-A film. Additionally, a reconstruction method of the immunoaffinity layer was presented by using oxygen plasma.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalSensors and Actuators, B: Chemical
Issue number2
Publication statusPublished - 2011 Jun 20

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea ( R15-2004-024-00000-0, 2009-008-1529, 2009-0073809, 20090082188 ) and by “System IC 2010” project of Ministry of Knowledge Economy and by the Seoul Research and Business Development Program ( 10816 ).

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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