Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery

Hyoung Joo Lee, Min Jeong Kang, Eun Young Lee, Yun Cho Sang, Hoguen Kim, Young-Ki Paik

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.

Original languageEnglish
Pages (from-to)3371-3381
Number of pages11
JournalProteomics
Volume8
Issue number16
DOIs
Publication statusPublished - 2008 Aug 1

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Biomarkers
Proteomics
Peptides
Phosphoproteins
Liquids
Fractionation
Hepatocellular Carcinoma
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Lee, Hyoung Joo ; Kang, Min Jeong ; Lee, Eun Young ; Sang, Yun Cho ; Kim, Hoguen ; Paik, Young-Ki. / Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery. In: Proteomics. 2008 ; Vol. 8, No. 16. pp. 3371-3381.
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Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery. / Lee, Hyoung Joo; Kang, Min Jeong; Lee, Eun Young; Sang, Yun Cho; Kim, Hoguen; Paik, Young-Ki.

In: Proteomics, Vol. 8, No. 16, 01.08.2008, p. 3371-3381.

Research output: Contribution to journalArticle

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