Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery

Hyoung Joo Lee, Min Jeong Kang, Eun Young Lee, Yun Cho Sang, Hoguen Kim, Young Ki Paik

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.

Original languageEnglish
Pages (from-to)3371-3381
Number of pages11
JournalProteomics
Volume8
Issue number16
DOIs
Publication statusPublished - 2008 Aug 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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