Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection

Maciej Antkowiak, Maria Leilani Torres-Mapa, Frank Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We demonstrate the advantages of a dynamic diffractive optical element, namely a spatial light modulator (SLM) for the controlled and enhanced optoinjection and phototransfection of mammalian cells with a femtosecond light source. The SLM provides full control over the lateral and axial positioning of the beam with submicron precision. Fast beam translation enables timesequenced irradiation, which is shown to enhance the optoinjection efficiency and alleviate the problem of exact beam positioning on the cell membrane. We show that irradiation in three axial positions doubles the number of viably optoinjected cells when compared with a single dose. The presented system also enables untargeted raster scan irradiation which provides a higher throughput transfection of adherent cells at the rate of 1 cell per second. Additionally, fluorescent imaging is used to demonstrate cell selective two-step gene therapy.

Original languageEnglish
Pages (from-to)696-705
Number of pages10
JournalJournal of Biophotonics
Volume3
Issue number10-11
DOIs
Publication statusPublished - 2010 Oct 1

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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