Application of metabolomics in prediction of lymph node metastasis in papillary thyroid carcinoma

Ji Won Seo, Kyunghwa Han, Jandee Lee, Eun Kyung Kim, Hee Jung Moon, Jung Hyun Yoon, Vivian Youngjean Park, Hyeon Man Baek, Jin Young Kwak

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose The aim of this study was to find useful metabolites to predict lymph node (LN) metastasis in patients with papillary thyroid cancer (PTC) through a metabolomics approach and investigate the potential role of metabolites as a novel prognostic marker. Materials and methods Fifty-two consecutive patients (median age: 41.5 years, range 15–74 years) were enrolled who underwent total thyroidectomy and central LN dissection with or without lateral LN dissection in Severance Hospital between October 2013 and July 2015. The study specimens were provided by the Severance Hospital Gene Bank, and consisted of PTC from each patient. The specimens were prepared for proton nuclear magnetic resonance (1H-NMR) spectroscopy. Spectral data by 1H-NMR spectroscopy were acquired, processed, and analyzed. Patients were grouped in three ways, according to the presence of LN metastasis, central LN metastasis and lateral LN metastasis. Chi-square test and the student t-test were used to analyze categorical variables and continuous variables, respectively. The Mann-Whitney U test was used for univariate analysis of metabolites. Orthogonal projections to latent structure discriminant analysis (OPLS-DA) was used for multivariate analysis to discriminate metabolic differences between the two groups. Results Among 52 patients, 32 had central LN metastasis and 19 had lateral LN metastasis. No clinical or histopathological characteristic was significantly different for all comparisons. On univariate analysis, no metabolite showed significant difference for all comparisons. On multivariate analysis, OPLS-DA did not discriminate the presence and absence of LN metastasis. Lactate was found to be the most promising metabolite. Conclusions No metabolite could discriminate the presence of LN metastasis. However, lactate was found to be the most promising metabolite for discrimination. Further studies with larger sample sizes are needed to elucidate significant metabolites which can indicate the presence of LN metastasis in patients with PTC.

Original languageEnglish
Article numbere0193883
JournalPloS one
Volume13
Issue number3
DOIs
Publication statusPublished - 2018 Mar

Fingerprint

Metabolomics
metabolomics
Metabolites
metastasis
carcinoma
lymph nodes
Lymph Nodes
Neoplasm Metastasis
prediction
metabolites
Dissection
Discriminant analysis
Magnetic Resonance Spectroscopy
Discriminant Analysis
nuclear magnetic resonance spectroscopy
Nuclear magnetic resonance spectroscopy
Lymph Node Excision
Lactic Acid
Nuclear magnetic resonance
Multivariate Analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Seo, Ji Won ; Han, Kyunghwa ; Lee, Jandee ; Kim, Eun Kyung ; Moon, Hee Jung ; Yoon, Jung Hyun ; Park, Vivian Youngjean ; Baek, Hyeon Man ; Kwak, Jin Young. / Application of metabolomics in prediction of lymph node metastasis in papillary thyroid carcinoma. In: PloS one. 2018 ; Vol. 13, No. 3.
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title = "Application of metabolomics in prediction of lymph node metastasis in papillary thyroid carcinoma",
abstract = "Purpose The aim of this study was to find useful metabolites to predict lymph node (LN) metastasis in patients with papillary thyroid cancer (PTC) through a metabolomics approach and investigate the potential role of metabolites as a novel prognostic marker. Materials and methods Fifty-two consecutive patients (median age: 41.5 years, range 15–74 years) were enrolled who underwent total thyroidectomy and central LN dissection with or without lateral LN dissection in Severance Hospital between October 2013 and July 2015. The study specimens were provided by the Severance Hospital Gene Bank, and consisted of PTC from each patient. The specimens were prepared for proton nuclear magnetic resonance (1H-NMR) spectroscopy. Spectral data by 1H-NMR spectroscopy were acquired, processed, and analyzed. Patients were grouped in three ways, according to the presence of LN metastasis, central LN metastasis and lateral LN metastasis. Chi-square test and the student t-test were used to analyze categorical variables and continuous variables, respectively. The Mann-Whitney U test was used for univariate analysis of metabolites. Orthogonal projections to latent structure discriminant analysis (OPLS-DA) was used for multivariate analysis to discriminate metabolic differences between the two groups. Results Among 52 patients, 32 had central LN metastasis and 19 had lateral LN metastasis. No clinical or histopathological characteristic was significantly different for all comparisons. On univariate analysis, no metabolite showed significant difference for all comparisons. On multivariate analysis, OPLS-DA did not discriminate the presence and absence of LN metastasis. Lactate was found to be the most promising metabolite. Conclusions No metabolite could discriminate the presence of LN metastasis. However, lactate was found to be the most promising metabolite for discrimination. Further studies with larger sample sizes are needed to elucidate significant metabolites which can indicate the presence of LN metastasis in patients with PTC.",
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Application of metabolomics in prediction of lymph node metastasis in papillary thyroid carcinoma. / Seo, Ji Won; Han, Kyunghwa; Lee, Jandee; Kim, Eun Kyung; Moon, Hee Jung; Yoon, Jung Hyun; Park, Vivian Youngjean; Baek, Hyeon Man; Kwak, Jin Young.

In: PloS one, Vol. 13, No. 3, e0193883, 03.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Application of metabolomics in prediction of lymph node metastasis in papillary thyroid carcinoma

AU - Seo, Ji Won

AU - Han, Kyunghwa

AU - Lee, Jandee

AU - Kim, Eun Kyung

AU - Moon, Hee Jung

AU - Yoon, Jung Hyun

AU - Park, Vivian Youngjean

AU - Baek, Hyeon Man

AU - Kwak, Jin Young

PY - 2018/3

Y1 - 2018/3

N2 - Purpose The aim of this study was to find useful metabolites to predict lymph node (LN) metastasis in patients with papillary thyroid cancer (PTC) through a metabolomics approach and investigate the potential role of metabolites as a novel prognostic marker. Materials and methods Fifty-two consecutive patients (median age: 41.5 years, range 15–74 years) were enrolled who underwent total thyroidectomy and central LN dissection with or without lateral LN dissection in Severance Hospital between October 2013 and July 2015. The study specimens were provided by the Severance Hospital Gene Bank, and consisted of PTC from each patient. The specimens were prepared for proton nuclear magnetic resonance (1H-NMR) spectroscopy. Spectral data by 1H-NMR spectroscopy were acquired, processed, and analyzed. Patients were grouped in three ways, according to the presence of LN metastasis, central LN metastasis and lateral LN metastasis. Chi-square test and the student t-test were used to analyze categorical variables and continuous variables, respectively. The Mann-Whitney U test was used for univariate analysis of metabolites. Orthogonal projections to latent structure discriminant analysis (OPLS-DA) was used for multivariate analysis to discriminate metabolic differences between the two groups. Results Among 52 patients, 32 had central LN metastasis and 19 had lateral LN metastasis. No clinical or histopathological characteristic was significantly different for all comparisons. On univariate analysis, no metabolite showed significant difference for all comparisons. On multivariate analysis, OPLS-DA did not discriminate the presence and absence of LN metastasis. Lactate was found to be the most promising metabolite. Conclusions No metabolite could discriminate the presence of LN metastasis. However, lactate was found to be the most promising metabolite for discrimination. Further studies with larger sample sizes are needed to elucidate significant metabolites which can indicate the presence of LN metastasis in patients with PTC.

AB - Purpose The aim of this study was to find useful metabolites to predict lymph node (LN) metastasis in patients with papillary thyroid cancer (PTC) through a metabolomics approach and investigate the potential role of metabolites as a novel prognostic marker. Materials and methods Fifty-two consecutive patients (median age: 41.5 years, range 15–74 years) were enrolled who underwent total thyroidectomy and central LN dissection with or without lateral LN dissection in Severance Hospital between October 2013 and July 2015. The study specimens were provided by the Severance Hospital Gene Bank, and consisted of PTC from each patient. The specimens were prepared for proton nuclear magnetic resonance (1H-NMR) spectroscopy. Spectral data by 1H-NMR spectroscopy were acquired, processed, and analyzed. Patients were grouped in three ways, according to the presence of LN metastasis, central LN metastasis and lateral LN metastasis. Chi-square test and the student t-test were used to analyze categorical variables and continuous variables, respectively. The Mann-Whitney U test was used for univariate analysis of metabolites. Orthogonal projections to latent structure discriminant analysis (OPLS-DA) was used for multivariate analysis to discriminate metabolic differences between the two groups. Results Among 52 patients, 32 had central LN metastasis and 19 had lateral LN metastasis. No clinical or histopathological characteristic was significantly different for all comparisons. On univariate analysis, no metabolite showed significant difference for all comparisons. On multivariate analysis, OPLS-DA did not discriminate the presence and absence of LN metastasis. Lactate was found to be the most promising metabolite. Conclusions No metabolite could discriminate the presence of LN metastasis. However, lactate was found to be the most promising metabolite for discrimination. Further studies with larger sample sizes are needed to elucidate significant metabolites which can indicate the presence of LN metastasis in patients with PTC.

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