Application of molecular techniques in biohydrogen production as a clean fuel

Gopalakrishnan Kumar, Thangavel Mathimani, Ramachandran Sivaramakrishnan, Sabarathinam Shanmugam, Shashi Kant Bhatia, Arivalagan Pugazhendhi

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


Considering the future energy demand and pollution to the environment, biohydrogen, a biofuel, produced from biological sources have garnered increased attention. The present review emphasis the various techniques and methods employed to enumerate the microbial community and enhancement of hydrogen production by dark fermentation. Notably, molecular techniques such as terminal restriction fragment length polymorphism (T-RFLP), quantitative real-time PCR (q-PCR), fluorescent in-situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE), ribosomal intergenic spacer analysis (RISA), and next generation sequencing (NGS) have been extensively discussed on identifying the microbial population in hydrogen production. Further, challenges and merits of the molecular techniques have been elaborated.

Original languageEnglish
Article number137795
JournalScience of the Total Environment
Publication statusPublished - 2020 Jun 20

Bibliographical note

Publisher Copyright:
© 2020 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution


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