Abstract
Surface Plasmon resonance (SPR) biosensor has been applied for the on-line monitoring of a target analyte in complex mixture by using specific interaction between antigens and antibodies. In this work, mainly for reusability of SPR biochip, parlyene thin film was applied as a linker layer on gold surface of the SPR biochip. Parylene is a polymer of p-xylene with good optical properties, chemical resistance from organic solvents and relatively high mechanical strength which are required for the linker layer of SPR biosensor. The SPR effect is known to be the occurrence of an evanescence field by the absorption of incident light at the range of 100nm from the sensor surface and we tested the feasibility of parylene layer of less than 100nm as a linker layer by observation of resonance angle change according to refractive index. Parylene film can be easily coated and removed by oxygen plasma treatment so that SPR biochip can be used repeatedly. The applicability of the parylene film was demonstrated for the detection of C-reactive protein which is widely used to check for inflammation.
| Original language | English |
|---|---|
| Pages (from-to) | 1035-1038 |
| Number of pages | 4 |
| Journal | Procedia Chemistry |
| Volume | 1 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 Sep 1 |
| Event | Eurosensors 23rd Conference - Lausanne, Switzerland Duration: 2009 Sep 6 → 2009 Sep 9 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Chemical Engineering(all)
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Application of parylene film as a linker layer of SPR biosensor. / Jeon, Byoung jin; Kim, Moo hwan; Pyun, Jae Chul.
In: Procedia Chemistry, Vol. 1, No. 1, 01.09.2009, p. 1035-1038.Research output: Contribution to journal › Conference article
TY - JOUR
T1 - Application of parylene film as a linker layer of SPR biosensor
AU - Jeon, Byoung jin
AU - Kim, Moo hwan
AU - Pyun, Jae Chul
PY - 2009/9/1
Y1 - 2009/9/1
N2 - Surface Plasmon resonance (SPR) biosensor has been applied for the on-line monitoring of a target analyte in complex mixture by using specific interaction between antigens and antibodies. In this work, mainly for reusability of SPR biochip, parlyene thin film was applied as a linker layer on gold surface of the SPR biochip. Parylene is a polymer of p-xylene with good optical properties, chemical resistance from organic solvents and relatively high mechanical strength which are required for the linker layer of SPR biosensor. The SPR effect is known to be the occurrence of an evanescence field by the absorption of incident light at the range of 100nm from the sensor surface and we tested the feasibility of parylene layer of less than 100nm as a linker layer by observation of resonance angle change according to refractive index. Parylene film can be easily coated and removed by oxygen plasma treatment so that SPR biochip can be used repeatedly. The applicability of the parylene film was demonstrated for the detection of C-reactive protein which is widely used to check for inflammation.
AB - Surface Plasmon resonance (SPR) biosensor has been applied for the on-line monitoring of a target analyte in complex mixture by using specific interaction between antigens and antibodies. In this work, mainly for reusability of SPR biochip, parlyene thin film was applied as a linker layer on gold surface of the SPR biochip. Parylene is a polymer of p-xylene with good optical properties, chemical resistance from organic solvents and relatively high mechanical strength which are required for the linker layer of SPR biosensor. The SPR effect is known to be the occurrence of an evanescence field by the absorption of incident light at the range of 100nm from the sensor surface and we tested the feasibility of parylene layer of less than 100nm as a linker layer by observation of resonance angle change according to refractive index. Parylene film can be easily coated and removed by oxygen plasma treatment so that SPR biochip can be used repeatedly. The applicability of the parylene film was demonstrated for the detection of C-reactive protein which is widely used to check for inflammation.
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U2 - 10.1016/j.proche.2009.07.258
DO - 10.1016/j.proche.2009.07.258
M3 - Conference article
VL - 1
SP - 1035
EP - 1038
JO - Procedia Chemistry
JF - Procedia Chemistry
SN - 1876-6196
IS - 1
ER -