Application of spectral SPR imaging for the surface analysis of C-reactive protein binding

Jong Seol Yuk, Duk Geun Hong, Hyo Il Jung, Kwon Soo Ha

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We investigated the surface characteristics of antibody binding on protein chips with resonance wavelength-based spectral surface plasmon resonance (SPR) imaging. The fluctuation of the resonance wavelengths for gold and dithiobis (succinimidyl propionate) (DTSP) array spots were less than 0.4 nm, which was good enough to analyze biomolecular interactions on the arrays. A serial process of biomolecular interactions from gold to anti-C-reactive protein (CRP) on the protein chips was monitored by the spectral SPR imaging. SPR images showed that distribution of anti-CRP binding onto the CRP surface was not uniform. Changes of color in the two-dimentional SPR images were explained by variations of the refractive index caused by differences in the amount of bound anti-CRP on protein chips. These results suggest that the spectral SPR imaging can be used as a novel tool for the analysis of surface distribution of protein binding with a label-free format.

Original languageEnglish
Pages (from-to)673-675
Number of pages3
JournalSensors and Actuators, B: Chemical
Volume119
Issue number2
DOIs
Publication statusPublished - 2006 Dec 7

Fingerprint

Surface analysis
Surface plasmon resonance
surface plasmon resonance
C-Reactive Protein
proteins
Proteins
Imaging techniques
Gold
chips
Wavelength
Labels
gold
Refractive index
Antibodies
Protein Binding
Color
antibodies
wavelengths
format
interactions

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Yuk, Jong Seol ; Hong, Duk Geun ; Jung, Hyo Il ; Ha, Kwon Soo. / Application of spectral SPR imaging for the surface analysis of C-reactive protein binding. In: Sensors and Actuators, B: Chemical. 2006 ; Vol. 119, No. 2. pp. 673-675.
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Application of spectral SPR imaging for the surface analysis of C-reactive protein binding. / Yuk, Jong Seol; Hong, Duk Geun; Jung, Hyo Il; Ha, Kwon Soo.

In: Sensors and Actuators, B: Chemical, Vol. 119, No. 2, 07.12.2006, p. 673-675.

Research output: Contribution to journalArticle

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