Application of spectral SPR imaging for the surface analysis of C-reactive protein binding

Jong Seol Yuk, Duk Geun Hong, Hyo Il Jung, Kwon Soo Ha

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


We investigated the surface characteristics of antibody binding on protein chips with resonance wavelength-based spectral surface plasmon resonance (SPR) imaging. The fluctuation of the resonance wavelengths for gold and dithiobis (succinimidyl propionate) (DTSP) array spots were less than 0.4 nm, which was good enough to analyze biomolecular interactions on the arrays. A serial process of biomolecular interactions from gold to anti-C-reactive protein (CRP) on the protein chips was monitored by the spectral SPR imaging. SPR images showed that distribution of anti-CRP binding onto the CRP surface was not uniform. Changes of color in the two-dimentional SPR images were explained by variations of the refractive index caused by differences in the amount of bound anti-CRP on protein chips. These results suggest that the spectral SPR imaging can be used as a novel tool for the analysis of surface distribution of protein binding with a label-free format.

Original languageEnglish
Pages (from-to)673-675
Number of pages3
JournalSensors and Actuators, B: Chemical
Issue number2
Publication statusPublished - 2006 Dec 7

Bibliographical note

Funding Information:
This work was supported in part by the grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (03-PJ10-PG6-GP01-0002).

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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