Application of SPR biosensor for medical diagnostics of human hepatitis B virus (hHBV)

J. W. Chung; S. D. Kim; R. Bernhardt; J. C. Pyun

doi:10.1016/j.snb.2005.03.055

Application of SPR biosensor for medical diagnostics of human hepatitis B virus (hHBV)

J. W. Chung, S. D. Kim, R. Bernhardt, J. C. Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

116 Citations (Scopus)

Abstract

The feasibility of a surface plasmon resonance (SPR) biosensor was tested for the medical diagnosis of human hepatitis B virus (hHBV). For the practical application of the SPR biosensor, the baseline stability and the dilution factor of serum samples was optimized. A standard curve was prepared using samples of HBV antibodies. In order to improve the sensitivity of the SPR biosensor, several amplification methods were tested using secondary antibodies, avidin-biotynylated antibodies and peroxidase-anti-peroxidase (PAP) complex. By applying the amplification methods, the detection limit of the SPR biosensor for the medical diagnosis was found to be very similar to the commercial ELISA kit for the diagnosis of hepatitis as a reference method.

Original language	English
Pages (from-to)	416-422
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	111-112
Issue number	SUPPL.
DOIs	https://doi.org/10.1016/j.snb.2005.03.055
Publication status	Published - 2005 Nov 11

Bibliographical note

Funding Information:
The authors would like to thank for the financial supports of Ministry of Science and Technology (MOST, Korea) and Korean Institute of Science and Technology (KIST, Korea).

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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abstract = "The feasibility of a surface plasmon resonance (SPR) biosensor was tested for the medical diagnosis of human hepatitis B virus (hHBV). For the practical application of the SPR biosensor, the baseline stability and the dilution factor of serum samples was optimized. A standard curve was prepared using samples of HBV antibodies. In order to improve the sensitivity of the SPR biosensor, several amplification methods were tested using secondary antibodies, avidin-biotynylated antibodies and peroxidase-anti-peroxidase (PAP) complex. By applying the amplification methods, the detection limit of the SPR biosensor for the medical diagnosis was found to be very similar to the commercial ELISA kit for the diagnosis of hepatitis as a reference method.",

author = "Chung, {J. W.} and Kim, {S. D.} and R. Bernhardt and Pyun, {J. C.}",

note = "Funding Information: The authors would like to thank for the financial supports of Ministry of Science and Technology (MOST, Korea) and Korean Institute of Science and Technology (KIST, Korea).",

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AU - Pyun, J. C.

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