Applications of stable, nonradioactive isotope tracers in in vivo human metabolic research

Il Young Kim, Sang-Hoon Suh, In Kyu Lee, Robert R. Wolfe

Research output: Contribution to journalReview article

22 Citations (Scopus)

Abstract

The human body is in a constant state of turnover, that is, being synthesized, broken down and/or converted to different compounds. The dynamic nature of in vivo kinetics of human metabolism at rest and in stressed conditions such as exercise and pathophysiological conditions such as diabetes and cancer can be quantitatively assessed with stable, nonradioactive isotope tracers in conjunction with gas or liquid chromatography mass spectrometry and modeling. Although measurements of metabolite concentrations have been useful as general indicators of one's health status, critical information on in vivo kinetics of metabolites such as rates of production, appearance or disappearance of metabolites are not provided. Over the past decades, stable, nonradioactive isotope tracers have been used to provide information on dynamics of specific metabolites. Stable isotope tracers can be used in conjunction with molecular and cellular biology tools, thereby providing an in-depth dynamic assessment of metabolic changes, as well as simultaneous investigation of the molecular basis for the observed kinetic responses. In this review, we will introduce basic principles of stable isotope methodology for tracing in vivo kinetics of human or animal metabolism with examples of quantifying certain aspects of in vivo kinetics of carbohydrate, lipid and protein metabolism.

Original languageEnglish
Article numbere203
JournalExperimental and Molecular Medicine
Volume48
DOIs
Publication statusPublished - 2016 Jan 15

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Metabolites
Isotopes
Enzyme kinetics
Metabolism
Research
Cytology
Molecular biology
Kinetics
Health Status Indicators
Liquid chromatography
Medical problems
Gas chromatography
Mass spectrometry
Animals
Human Body
Lipid Metabolism
Gas Chromatography-Mass Spectrometry
Carbohydrates
Health
Cell Biology

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

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Applications of stable, nonradioactive isotope tracers in in vivo human metabolic research. / Kim, Il Young; Suh, Sang-Hoon; Lee, In Kyu; Wolfe, Robert R.

In: Experimental and Molecular Medicine, Vol. 48, e203, 15.01.2016.

Research output: Contribution to journalReview article

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