Aptamer-based microspheres for highly sensitive protein detection using fluorescently-labeled DNA nanostructures

Daehoon Han, Jinkee Hong, Hyun Cheol Kim, Jong Hwan Sung, Jong Bum Lee

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Many highly sensitive protein detection techniques have been developed and have played an important role in the analysis of proteins. Herein, we report a novel technique that can detect proteins sensitively and effectively using aptamer-based DNA nanostructures. Thrombin was used as a target protein and aptamer was used to capture fluorescent dye-labeled DNA nanobarcodes or thrombin on a microsphere. The captured DNA nanobarcodes were replaced by a thrombin and aptamer interaction. The detection ability of this approach was confirmed by flow cytometry with different concentrations of thrombin. Our detection method has great potential for rapid and simple protein detection with a variety of aptamers.

Original languageEnglish
Pages (from-to)7259-7263
Number of pages5
JournalJournal of Nanoscience and Nanotechnology
Volume13
Issue number11
DOIs
Publication statusPublished - 2013 Nov

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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