Aptamer-conjugated gold nanorod for photothermal ablation of EGFR-overexpressed epithelial cancer

Jihye Choi, Yeonji Park, Eun Bi Choi, Hyun Ouk Kim, Yoochan Hong, Sung Ho Ryu, Jung Hwan Lee, Jin Suck Suh, Jaemoon Yang, Yong Min Huh, Seungjoo Haam

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biomarker-specific photothermal nanoparticles that can efficiently sense the markers, which are overexpressed in distinguished adenocarcinomas, has attracted much interest in an aspect of efficacy increase of cancer treatment. In this study, we demonstrated a promising prospect of smart photothermal therapy agent employing anti-epidermal growth factor receptor aptamer (AptEGFR)-conjugated polyethylene glycol (PEG)layted gold nanorods (AptEGFR-PGNRs). The cetyltrimethylammonium bromide bilayer on GNRs was replaced with heterobifunctional polyethylene glycol (COOHPEG- SH) not only to serve as a biocompatible stabilizer and but also to conjugate AptEGFR. Subsequently, to direct photothermal therapy agent toward epithelial cancer cells, the carboxylated PEGylated GNRs (PGNRs) were further functionalized with AptEGFR using carbodiimide chemistry. And then, to assess the potential as biomarker-specific photothermal therapy agent of synthesized AptEGFR-PGNRs, the optical properties, biocompatibility, colloidal stability, binding affinity and epicellial cancer cell killing efficacy in vitro/in vivo under NIR laser irradiation were investigated. As a results, AptEGFR-PGNRs exhibit excellent tumor targeting ability and feasibility of effective photothermal ablation cancer therapy.

Original languageEnglish
Title of host publicationNano-Bio Sensing, Imaging, and Spectroscopy
DOIs
Publication statusPublished - 2013 Sep 17
EventSPIE Nano-Bio Sensing, Imaging, and Spectroscopy - Jeju, Korea, Republic of
Duration: 2013 Feb 202013 Feb 23

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8879
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Other

OtherSPIE Nano-Bio Sensing, Imaging, and Spectroscopy
CountryKorea, Republic of
CityJeju
Period13/2/2013/2/23

Fingerprint

Nanorods
Ablation
Gold
nanorods
ablation
Growth Factors
Cancer
cancer
Epidermal Growth Factor Receptor
Receptor
gold
Therapy
therapy
biomarkers
Biomarkers
Polyethylene glycols
Efficacy
glycols
polyethylenes
Cells

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Choi, J., Park, Y., Choi, E. B., Kim, H. O., Hong, Y., Ryu, S. H., ... Haam, S. (2013). Aptamer-conjugated gold nanorod for photothermal ablation of EGFR-overexpressed epithelial cancer. In Nano-Bio Sensing, Imaging, and Spectroscopy [88790S] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8879). https://doi.org/10.1117/12.2018719
Choi, Jihye ; Park, Yeonji ; Choi, Eun Bi ; Kim, Hyun Ouk ; Hong, Yoochan ; Ryu, Sung Ho ; Lee, Jung Hwan ; Suh, Jin Suck ; Yang, Jaemoon ; Huh, Yong Min ; Haam, Seungjoo. / Aptamer-conjugated gold nanorod for photothermal ablation of EGFR-overexpressed epithelial cancer. Nano-Bio Sensing, Imaging, and Spectroscopy. 2013. (Proceedings of SPIE - The International Society for Optical Engineering).
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title = "Aptamer-conjugated gold nanorod for photothermal ablation of EGFR-overexpressed epithelial cancer",
abstract = "Biomarker-specific photothermal nanoparticles that can efficiently sense the markers, which are overexpressed in distinguished adenocarcinomas, has attracted much interest in an aspect of efficacy increase of cancer treatment. In this study, we demonstrated a promising prospect of smart photothermal therapy agent employing anti-epidermal growth factor receptor aptamer (AptEGFR)-conjugated polyethylene glycol (PEG)layted gold nanorods (AptEGFR-PGNRs). The cetyltrimethylammonium bromide bilayer on GNRs was replaced with heterobifunctional polyethylene glycol (COOHPEG- SH) not only to serve as a biocompatible stabilizer and but also to conjugate AptEGFR. Subsequently, to direct photothermal therapy agent toward epithelial cancer cells, the carboxylated PEGylated GNRs (PGNRs) were further functionalized with AptEGFR using carbodiimide chemistry. And then, to assess the potential as biomarker-specific photothermal therapy agent of synthesized AptEGFR-PGNRs, the optical properties, biocompatibility, colloidal stability, binding affinity and epicellial cancer cell killing efficacy in vitro/in vivo under NIR laser irradiation were investigated. As a results, AptEGFR-PGNRs exhibit excellent tumor targeting ability and feasibility of effective photothermal ablation cancer therapy.",
author = "Jihye Choi and Yeonji Park and Choi, {Eun Bi} and Kim, {Hyun Ouk} and Yoochan Hong and Ryu, {Sung Ho} and Lee, {Jung Hwan} and Suh, {Jin Suck} and Jaemoon Yang and Huh, {Yong Min} and Seungjoo Haam",
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Choi, J, Park, Y, Choi, EB, Kim, HO, Hong, Y, Ryu, SH, Lee, JH, Suh, JS, Yang, J, Huh, YM & Haam, S 2013, Aptamer-conjugated gold nanorod for photothermal ablation of EGFR-overexpressed epithelial cancer. in Nano-Bio Sensing, Imaging, and Spectroscopy., 88790S, Proceedings of SPIE - The International Society for Optical Engineering, vol. 8879, SPIE Nano-Bio Sensing, Imaging, and Spectroscopy, Jeju, Korea, Republic of, 13/2/20. https://doi.org/10.1117/12.2018719

Aptamer-conjugated gold nanorod for photothermal ablation of EGFR-overexpressed epithelial cancer. / Choi, Jihye; Park, Yeonji; Choi, Eun Bi; Kim, Hyun Ouk; Hong, Yoochan; Ryu, Sung Ho; Lee, Jung Hwan; Suh, Jin Suck; Yang, Jaemoon; Huh, Yong Min; Haam, Seungjoo.

Nano-Bio Sensing, Imaging, and Spectroscopy. 2013. 88790S (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8879).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Ryu, Sung Ho

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AU - Huh, Yong Min

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Choi J, Park Y, Choi EB, Kim HO, Hong Y, Ryu SH et al. Aptamer-conjugated gold nanorod for photothermal ablation of EGFR-overexpressed epithelial cancer. In Nano-Bio Sensing, Imaging, and Spectroscopy. 2013. 88790S. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.2018719