Aptamer-functionalized capacitance sensors for real-time monitoring of bacterial growth and antibiotic susceptibility

Namgyeong Jo, Bongjun Kim, Sun Mi Lee, Jeseung Oh, In Ho Park, Kook Jin Lim, Jeon Soo Shin, Kyung Hwa Yoo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To prevent spread of infection and antibiotic resistance, fast and accurate diagnosis of bacterial infection and subsequent administration of antimicrobial agents are important. However, conventional methods for bacterial detection and antibiotic susceptibility testing (AST) require more than two days, leading to delays that have contributed to an increase in antibiotic-resistant bacteria. Here, we report an aptamer-functionalized capacitance sensor array that can monitor bacterial growth and antibiotic susceptibility in real-time. While E. coli and S. aureus were cultured, the capacitance increased over time, and apparent bacterial growth curves were observed even when 10 CFU/mL bacteria was inoculated. Furthermore, because of the selectivity of aptamers, bacteria could be identified within 1 h using the capacitance sensor array functionalized with aptamers. In addition to bacterial growth, antibiotic susceptibility could be monitored in real-time. When bacteria were treated with antibiotics above the minimum inhibitory concentration (MIC), the capacitance decreased because the bacterial growth was inhibited. These results demonstrate that the aptamer-functionalized capacitance sensor array might be applied for rapid ASTs.

Original languageEnglish
Pages (from-to)164-170
Number of pages7
JournalBiosensors and Bioelectronics
Volume102
DOIs
Publication statusPublished - 2018 Apr 15

Fingerprint

Antibiotics
Capacitance
Anti-Bacterial Agents
Monitoring
Bacteria
Sensors
Sensor arrays
Growth
Antimicrobial agents
Microbial Sensitivity Tests
Microbial Drug Resistance
Anti-Infective Agents
Bacterial Infections
Escherichia coli
Infection
Testing

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Jo, Namgyeong ; Kim, Bongjun ; Lee, Sun Mi ; Oh, Jeseung ; Park, In Ho ; Jin Lim, Kook ; Shin, Jeon Soo ; Yoo, Kyung Hwa. / Aptamer-functionalized capacitance sensors for real-time monitoring of bacterial growth and antibiotic susceptibility. In: Biosensors and Bioelectronics. 2018 ; Vol. 102. pp. 164-170.
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Aptamer-functionalized capacitance sensors for real-time monitoring of bacterial growth and antibiotic susceptibility. / Jo, Namgyeong; Kim, Bongjun; Lee, Sun Mi; Oh, Jeseung; Park, In Ho; Jin Lim, Kook; Shin, Jeon Soo; Yoo, Kyung Hwa.

In: Biosensors and Bioelectronics, Vol. 102, 15.04.2018, p. 164-170.

Research output: Contribution to journalArticle

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