Aptamer-modified magnetic nanoprobe for molecular MR imaging of VEGFR2 on angiogenic vasculature

Bongjune Kim, Jaemoon Yang, Myeonghwan Hwang, Jihye Choi, Hyun Ouk Kim, Eunji Jang, Jung Hwan Lee, Sung Ho Ryu, Jinsuck Suh, yongmin Huh, Seungjoo Haam

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Nucleic acid-based aptamers have been developed for the specific delivery of diagnostic nanoprobes. Here, we introduce a new class of smart imaging nanoprobe, which is based on hybridization of a magnetic nanocrystal with a specific aptamer for specific detection of the angiogenic vasculature of glioblastoma via magnetic resonance (MR) imaging. The magnetic nanocrystal imaging core was synthesized using the thermal decomposition method and enveloped by carboxyl polysorbate 80 for water solubilization and conjugation of the targeting moiety. Subsequently, the surface of the carboxylated magnetic nanocrystal was modified with amine-functionalized aptamers that specifically bind to the vascular growth factor receptor 2 (VEGFR2) that is overexpressed on angiogenic vessels. To assess the targeted imaging potential of the aptamer-conjugated magnetic nanocrystal for VEGFR2 markers, the magnetic properties and MR imaging sensitivity were investigated using the orthotopic glioblastoma mouse model. In in vivo tests, the aptamer-conjugated magnetic nanocrystal effectively targeted VEGFR2 and demonstrated excellent MR imaging sensitivity with no cytotoxicity.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalNanoscale Research Letters
Volume8
Issue number1
DOIs
Publication statusPublished - 2013 Nov 14

Fingerprint

Nanoprobes
Magnetic resonance
magnetic resonance
nanocrystals
Nanocrystals
Imaging techniques
sensitivity
nucleic acids
conjugation
Growth Factor Receptors
Polysorbates
markers
Nucleic acids
thermal decomposition
vessels
mice
Cytotoxicity
delivery
amines
Nucleic Acids

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Kim, Bongjune ; Yang, Jaemoon ; Hwang, Myeonghwan ; Choi, Jihye ; Kim, Hyun Ouk ; Jang, Eunji ; Lee, Jung Hwan ; Ryu, Sung Ho ; Suh, Jinsuck ; Huh, yongmin ; Haam, Seungjoo. / Aptamer-modified magnetic nanoprobe for molecular MR imaging of VEGFR2 on angiogenic vasculature. In: Nanoscale Research Letters. 2013 ; Vol. 8, No. 1. pp. 1-10.
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Aptamer-modified magnetic nanoprobe for molecular MR imaging of VEGFR2 on angiogenic vasculature. / Kim, Bongjune; Yang, Jaemoon; Hwang, Myeonghwan; Choi, Jihye; Kim, Hyun Ouk; Jang, Eunji; Lee, Jung Hwan; Ryu, Sung Ho; Suh, Jinsuck; Huh, yongmin; Haam, Seungjoo.

In: Nanoscale Research Letters, Vol. 8, No. 1, 14.11.2013, p. 1-10.

Research output: Contribution to journalArticle

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