Aptamer-Modified Magnetic Nanosensitizer for In Vivo MR Imaging of HER2-Expressing Cancer

Dan Heo, Minhee Ku, Jung Hoon Kim, Jaemoon Yang, Jin Suck Suh

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Abstract

The aim of this study was the development of a human epidermal growth factor receptor 2 (HER2)-targetable contrast agent for magnetic resonance imaging (MRI) with a high magnetic sensitivity. An anti-HER2 aptamer-modified magnetic nanosensitizer (AptHER2-MNS) was prepared by conjugation with 5′-thiol-modified aptamers and maleimidylated magnetic nanocrystals (MNCs). The physicochemical characteristics and targeting ability of AptHER2-MNS were confirmed, and the binding affinity (Kd) onto HER2 protein of AptHER2-MNS was 0.57 ± 0.26 nM. In vivo MRI contrast enhancement ability was also verified at HER2+ cancer cell (NIH3T6.7)-xenograft mouse models (n = 3) at 3T clinical MRI instrument. The control experiment was carried out using non-labeled MNCs. The results indicated that up to 150% contrast enhancement was achieved at the tumor region in the T2-weighted MR images after the injection of the AptHER2-MNS agent in mice that received the NIH3T6.7 cells.

Original languageEnglish
Article number288
JournalNanoscale Research Letters
Volume13
DOIs
Publication statusPublished - 2018

Bibliographical note

Funding Information:
This study was funded by the National Research Foundation grant funded by the Korean government, Ministry of Education and Science Technology (2015R1A2A1A05001887, 2017R1C1B2010867), the Korea government, Ministry of Trade, Industry and Energy (10047677), the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (HI17C2586, H17C1491, H16C0179), funded by the Ministry of Health & Welfare, and by a faculty research grant of Yonsei University College of Medicine for (6-2014-0032, 2014-32-0022), Republic of Korea.

Funding Information:
The authors gratefully acknowledge the financial support given for this work by the National Research Foundation grant funded by the Korean government, Ministry of Education and Science Technology (2015R1A2A1A05001887, 2017R1C1B2010867), the Korea government, Ministry of Trade, Industry and Energy (10047677), the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (HI17C2586, H17C1491, H16C0179), funded by the Ministry of Health & Welfare, and by a faculty research grant of Yonsei University College of Medicine for (6-2014-0032, 2014-32-0022), Republic of Korea.

Funding Information:
The authors gratefully acknowledge the financial support given for this work by the National Research Foundation grant funded by the Korean government, Ministry of Education and Science Technology (2015R1A2A1A05001887, 2017R1C1B2010867), the Korea government, Ministry of Trade, Industry and Energy (10047677), the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (HI17C2586, H17C1491, H16C0179), funded by the Ministry of Health & Welfare, and by a faculty research grant of Yonsei University College of Medicine for (6-2014-0032, 2014-32-0022), Republic of Korea. This study was funded by the National Research Foundation grant funded by the Korean government, Ministry of Education and Science Technology (2015R1A2A1A05001887, 2017R1C1B2010867), the Korea government, Ministry of Trade, Industry and Energy (10047677), the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (HI17C2586, H17C1491, H16C0179), funded by the Ministry of Health & Welfare, and by a faculty research grant of Yonsei University College of Medicine for (6-2014-0032, 2014-32-0022), Republic of Korea. The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics

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