Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response

Min Yong Ahn; Tae Rin Oh; Dong Hye Seo; Jong Hum Kim; Na Hyun Cho; Woo Taek Kim

doi:10.1016/j.jplph.2018.08.010

Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response

Min Yong Ahn, Tae Rin Oh, Dong Hye Seo, Jong Hum Kim, Na Hyun Cho, Woo Taek Kim

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

AtUBC32, AtUBC33, and AtUBC34 comprise Arabidopsis group XIV E2 ubiquitin-conjugating enzymes. Yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays revealed that group XIV E2s are interacting partners of the U-box-type E3 ligase PUB19, a negative regulator of drought stress response. These three AtUBCs are co-localized with PUB19 to the punctae-like structures, most of which reside on the endoplasmic reticulum membrane of tobacco leaf cells. Suppression of AtUBC32, AtUBC33, and AtUBC34 resulted in increased abscisic acid-mediated stomatal closure and tolerance to drought stress. These results indicate that Arabidopsis group XIV E2s play negative roles in drought stress response.

Original language	English
Pages (from-to)	73-79
Number of pages	7
Journal	Journal of Plant Physiology
Volume	230
DOIs	https://doi.org/10.1016/j.jplph.2018.08.010
Publication status	Published - 2018 Nov

Bibliographical note

Funding Information:
This work was supported by the grants from the National Research Foundation (Mid-Career Researcher Project No. NRF-2017R1A2B2006750) and the Next-Generation BioGreen 21 Program for Agriculture and Technology Development (SSAC Project No. PJ01334901 funded by the Rural Development Administration), Republic of Korea, to WTK.

Funding Information:
This work was supported by the grants from the National Research Foundation (Mid-Career Researcher Project No. NRF-2017R1A2B2006750) and the Next-Generation BioGreen 21 Program for Agriculture and Technology Development (SSAC Project No. PJ01334901 funded by the Rural Development Administration ), Republic of Korea, to WTK.

Publisher Copyright:
© 2018 Elsevier GmbH

All Science Journal Classification (ASJC) codes

Physiology
Agronomy and Crop Science
Plant Science

Access to Document

10.1016/j.jplph.2018.08.010

Cite this

@article{802d32c6df60449e9a6ead0b1e8b58bc,

title = "Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response",

abstract = "AtUBC32, AtUBC33, and AtUBC34 comprise Arabidopsis group XIV E2 ubiquitin-conjugating enzymes. Yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays revealed that group XIV E2s are interacting partners of the U-box-type E3 ligase PUB19, a negative regulator of drought stress response. These three AtUBCs are co-localized with PUB19 to the punctae-like structures, most of which reside on the endoplasmic reticulum membrane of tobacco leaf cells. Suppression of AtUBC32, AtUBC33, and AtUBC34 resulted in increased abscisic acid-mediated stomatal closure and tolerance to drought stress. These results indicate that Arabidopsis group XIV E2s play negative roles in drought stress response.",

author = "Ahn, {Min Yong} and Oh, {Tae Rin} and Seo, {Dong Hye} and Kim, {Jong Hum} and Cho, {Na Hyun} and Kim, {Woo Taek}",

note = "Funding Information: This work was supported by the grants from the National Research Foundation (Mid-Career Researcher Project No. NRF-2017R1A2B2006750) and the Next-Generation BioGreen 21 Program for Agriculture and Technology Development (SSAC Project No. PJ01334901 funded by the Rural Development Administration), Republic of Korea, to WTK. Funding Information: This work was supported by the grants from the National Research Foundation (Mid-Career Researcher Project No. NRF-2017R1A2B2006750) and the Next-Generation BioGreen 21 Program for Agriculture and Technology Development (SSAC Project No. PJ01334901 funded by the Rural Development Administration ), Republic of Korea, to WTK. Publisher Copyright: {\textcopyright} 2018 Elsevier GmbH",

year = "2018",

month = nov,

doi = "10.1016/j.jplph.2018.08.010",

language = "English",

volume = "230",

pages = "73--79",

journal = "Z. PFLANZENPHYSIOL.",

issn = "0176-1617",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

}

TY - JOUR

T1 - Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response

AU - Ahn, Min Yong

AU - Oh, Tae Rin

AU - Seo, Dong Hye

AU - Kim, Jong Hum

AU - Cho, Na Hyun

AU - Kim, Woo Taek

N1 - Funding Information: This work was supported by the grants from the National Research Foundation (Mid-Career Researcher Project No. NRF-2017R1A2B2006750) and the Next-Generation BioGreen 21 Program for Agriculture and Technology Development (SSAC Project No. PJ01334901 funded by the Rural Development Administration), Republic of Korea, to WTK. Funding Information: This work was supported by the grants from the National Research Foundation (Mid-Career Researcher Project No. NRF-2017R1A2B2006750) and the Next-Generation BioGreen 21 Program for Agriculture and Technology Development (SSAC Project No. PJ01334901 funded by the Rural Development Administration ), Republic of Korea, to WTK. Publisher Copyright: © 2018 Elsevier GmbH

PY - 2018/11

Y1 - 2018/11

N2 - AtUBC32, AtUBC33, and AtUBC34 comprise Arabidopsis group XIV E2 ubiquitin-conjugating enzymes. Yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays revealed that group XIV E2s are interacting partners of the U-box-type E3 ligase PUB19, a negative regulator of drought stress response. These three AtUBCs are co-localized with PUB19 to the punctae-like structures, most of which reside on the endoplasmic reticulum membrane of tobacco leaf cells. Suppression of AtUBC32, AtUBC33, and AtUBC34 resulted in increased abscisic acid-mediated stomatal closure and tolerance to drought stress. These results indicate that Arabidopsis group XIV E2s play negative roles in drought stress response.

AB - AtUBC32, AtUBC33, and AtUBC34 comprise Arabidopsis group XIV E2 ubiquitin-conjugating enzymes. Yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays revealed that group XIV E2s are interacting partners of the U-box-type E3 ligase PUB19, a negative regulator of drought stress response. These three AtUBCs are co-localized with PUB19 to the punctae-like structures, most of which reside on the endoplasmic reticulum membrane of tobacco leaf cells. Suppression of AtUBC32, AtUBC33, and AtUBC34 resulted in increased abscisic acid-mediated stomatal closure and tolerance to drought stress. These results indicate that Arabidopsis group XIV E2s play negative roles in drought stress response.

UR - http://www.scopus.com/inward/record.url?scp=85052903654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052903654&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2018.08.010

DO - 10.1016/j.jplph.2018.08.010

M3 - Article

C2 - 30193177

AN - SCOPUS:85052903654

SN - 0176-1617

VL - 230

SP - 73

EP - 79

JO - Z. PFLANZENPHYSIOL.

JF - Z. PFLANZENPHYSIOL.

ER -

Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this