AtUBC32, AtUBC33, and AtUBC34 comprise Arabidopsis group XIV E2 ubiquitin-conjugating enzymes. Yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays revealed that group XIV E2s are interacting partners of the U-box-type E3 ligase PUB19, a negative regulator of drought stress response. These three AtUBCs are co-localized with PUB19 to the punctae-like structures, most of which reside on the endoplasmic reticulum membrane of tobacco leaf cells. Suppression of AtUBC32, AtUBC33, and AtUBC34 resulted in increased abscisic acid-mediated stomatal closure and tolerance to drought stress. These results indicate that Arabidopsis group XIV E2s play negative roles in drought stress response.
Bibliographical noteFunding Information:
This work was supported by the grants from the National Research Foundation (Mid-Career Researcher Project No. NRF-2017R1A2B2006750) and the Next-Generation BioGreen 21 Program for Agriculture and Technology Development (SSAC Project No. PJ01334901 funded by the Rural Development Administration ), Republic of Korea, to WTK.
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science