Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response

Min Yong Ahn, Tae Rin Oh, Dong Hye Seo, Jong Hum Kim, Na Hyun Cho, Woo Taek Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

AtUBC32, AtUBC33, and AtUBC34 comprise Arabidopsis group XIV E2 ubiquitin-conjugating enzymes. Yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays revealed that group XIV E2s are interacting partners of the U-box-type E3 ligase PUB19, a negative regulator of drought stress response. These three AtUBCs are co-localized with PUB19 to the punctae-like structures, most of which reside on the endoplasmic reticulum membrane of tobacco leaf cells. Suppression of AtUBC32, AtUBC33, and AtUBC34 resulted in increased abscisic acid-mediated stomatal closure and tolerance to drought stress. These results indicate that Arabidopsis group XIV E2s play negative roles in drought stress response.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalJournal of Plant Physiology
Volume230
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

Ubiquitin-Conjugating Enzymes
ubiquitin-protein ligase
Droughts
Arabidopsis
stress response
water stress
Abscisic Acid
Ubiquitin-Protein Ligases
ligases
Endoplasmic Reticulum
endoplasmic reticulum
Tobacco
abscisic acid
tobacco
Yeasts
Fluorescence
fluorescence
yeasts
Membranes
assays

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

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title = "Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response",
abstract = "AtUBC32, AtUBC33, and AtUBC34 comprise Arabidopsis group XIV E2 ubiquitin-conjugating enzymes. Yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays revealed that group XIV E2s are interacting partners of the U-box-type E3 ligase PUB19, a negative regulator of drought stress response. These three AtUBCs are co-localized with PUB19 to the punctae-like structures, most of which reside on the endoplasmic reticulum membrane of tobacco leaf cells. Suppression of AtUBC32, AtUBC33, and AtUBC34 resulted in increased abscisic acid-mediated stomatal closure and tolerance to drought stress. These results indicate that Arabidopsis group XIV E2s play negative roles in drought stress response.",
author = "Ahn, {Min Yong} and Oh, {Tae Rin} and Seo, {Dong Hye} and Kim, {Jong Hum} and Cho, {Na Hyun} and Kim, {Woo Taek}",
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Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response. / Ahn, Min Yong; Oh, Tae Rin; Seo, Dong Hye; Kim, Jong Hum; Cho, Na Hyun; Kim, Woo Taek.

In: Journal of Plant Physiology, Vol. 230, 01.11.2018, p. 73-79.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Arabidopsis group XIV ubiquitin-conjugating enzymes AtUBC32, AtUBC33, and AtUBC34 play negative roles in drought stress response

AU - Ahn, Min Yong

AU - Oh, Tae Rin

AU - Seo, Dong Hye

AU - Kim, Jong Hum

AU - Cho, Na Hyun

AU - Kim, Woo Taek

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AB - AtUBC32, AtUBC33, and AtUBC34 comprise Arabidopsis group XIV E2 ubiquitin-conjugating enzymes. Yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays revealed that group XIV E2s are interacting partners of the U-box-type E3 ligase PUB19, a negative regulator of drought stress response. These three AtUBCs are co-localized with PUB19 to the punctae-like structures, most of which reside on the endoplasmic reticulum membrane of tobacco leaf cells. Suppression of AtUBC32, AtUBC33, and AtUBC34 resulted in increased abscisic acid-mediated stomatal closure and tolerance to drought stress. These results indicate that Arabidopsis group XIV E2s play negative roles in drought stress response.

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