Arabidopsis small rubber particle protein homolog srps play dual roles as positive factors for tissue growth and development and in drought stress responses

Eun Yu Kim, Ki Youl Park, Young Sam Seo, Woo Taek Kim

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Lipid droplets (LDs) act as repositories for fatty acids and sterols, which are used for various cellular processes such as energy production and membrane and hormone synthesis. LD-associated proteins play important roles in seed development and germination, but their functions in postgermination growth are not well understood. Arabidopsis (Arabidopsis thaliana) contains three SRP homologs (SRP1, SRP2, and SRP3) that share sequence identities with small rubber particle proteins of the rubber tree (Hevea brasiliensis). In this report, the possible cellular roles of SRPs in postgermination growth and the drought tolerance response were investigated. Arabidopsis SRPs appeared to be LD-associated proteins and displayed polymerization properties in vivo and in vitro. SRP-overexpressing transgenic Arabidopsis plants (35S:SRP1, 35S:SRP2, and 35S:SRP3) exhibited higher vegetative and reproductive growth and markedly better tolerance to drought stress than wild-type Arabidopsis. In addition, constitutive over-expression of SRPs resulted in increased numbers of large LDs in postgermination seedlings. In contrast, single (srp1, 35S:SRP2-RNAi, and srp3) and triple (35S:SRP2-RNAi/srp1srp3) loss-of-function mutant lines exhibited the opposite phenotypes. Our results suggest that Arabidopsis SRPs play dual roles as positive factors in postgermination growth and the drought stress tolerance response. The possible relationships between LD-associated proteins and the drought stress response are discussed.

Original languageEnglish
Pages (from-to)2494-2510
Number of pages17
JournalPlant physiology
Volume170
Issue number4
DOIs
Publication statusPublished - 2016 Apr 1

Fingerprint

Droughts
Rubber
Thromboplastin
rubber
Growth and Development
Arabidopsis
droplets
stress response
growth and development
water stress
lipids
Hevea
Hevea brasiliensis
Proteins
proteins
drought tolerance
Growth
RNA Interference
seed development
polymerization

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

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title = "Arabidopsis small rubber particle protein homolog srps play dual roles as positive factors for tissue growth and development and in drought stress responses",
abstract = "Lipid droplets (LDs) act as repositories for fatty acids and sterols, which are used for various cellular processes such as energy production and membrane and hormone synthesis. LD-associated proteins play important roles in seed development and germination, but their functions in postgermination growth are not well understood. Arabidopsis (Arabidopsis thaliana) contains three SRP homologs (SRP1, SRP2, and SRP3) that share sequence identities with small rubber particle proteins of the rubber tree (Hevea brasiliensis). In this report, the possible cellular roles of SRPs in postgermination growth and the drought tolerance response were investigated. Arabidopsis SRPs appeared to be LD-associated proteins and displayed polymerization properties in vivo and in vitro. SRP-overexpressing transgenic Arabidopsis plants (35S:SRP1, 35S:SRP2, and 35S:SRP3) exhibited higher vegetative and reproductive growth and markedly better tolerance to drought stress than wild-type Arabidopsis. In addition, constitutive over-expression of SRPs resulted in increased numbers of large LDs in postgermination seedlings. In contrast, single (srp1, 35S:SRP2-RNAi, and srp3) and triple (35S:SRP2-RNAi/srp1srp3) loss-of-function mutant lines exhibited the opposite phenotypes. Our results suggest that Arabidopsis SRPs play dual roles as positive factors in postgermination growth and the drought stress tolerance response. The possible relationships between LD-associated proteins and the drought stress response are discussed.",
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Arabidopsis small rubber particle protein homolog srps play dual roles as positive factors for tissue growth and development and in drought stress responses. / Kim, Eun Yu; Park, Ki Youl; Seo, Young Sam; Kim, Woo Taek.

In: Plant physiology, Vol. 170, No. 4, 01.04.2016, p. 2494-2510.

Research output: Contribution to journalArticle

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