Aristolactam BII of saururus chinensis attenuates glutamate-induced neurotoxicity in rat cortical cultures probably by inhibiting nitric oxide production

So Ra Kim, Sang Hyun Sung, So Young Kang, Kyung Ah Koo, Seung Hyun Kim, Choong Je Ma, Heum Sook Lee, Mi Jung Park, Young Choong Kim

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Saurolactam and aristolactam BII, aristolactam-type alkaloids isolated from the aerial part of Saururus chinensis (Lour.) Ball (Saururaceae), showed significant neuroprotective activity against glutamate-induced toxicity in primary cultured rat cortical cells. The action mechanism of aristolactam BII, the more potent neuroprotective compound, was investigated using primary cultures of rat cortical cells as an in vitro system. Aristolactam BII attenuated glutamate-induced neurotoxicity significantly when it was added immediately or up to 9 h after the excitotoxic glutamate challenge. The alkaloid could not protect cultured neuronal cells from neurotoxicity induced by kainic acid or N-methyl-D-aspartate in a pre-treatment paradigm. However, aristolactam BII successfully reduced the overproduction of nitric oxide and the level of cellular peroxide in cultured neurons when it was treated as a post-treatment paradigm. These results may suggest that aristolactam BII exerts its significant neuroprotective effects on glutamate-injured primary cultures of rat cortical cells by directly inhibiting the production of nitric oxide.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalPlanta Medica
Volume70
Issue number5
DOIs
Publication statusPublished - 2004 May 1

Fingerprint

Saururaceae
Rats
Glutamic Acid
Nitric Oxide
Alkaloids
Kainic Acid
Peroxides
Neuroprotective Agents
N-Methylaspartate
Neurons
Toxicity
Cultured Cells
aristolactam BII
Antennas

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

Kim, So Ra ; Sung, Sang Hyun ; Kang, So Young ; Koo, Kyung Ah ; Kim, Seung Hyun ; Ma, Choong Je ; Lee, Heum Sook ; Park, Mi Jung ; Kim, Young Choong. / Aristolactam BII of saururus chinensis attenuates glutamate-induced neurotoxicity in rat cortical cultures probably by inhibiting nitric oxide production. In: Planta Medica. 2004 ; Vol. 70, No. 5. pp. 391-396.
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Aristolactam BII of saururus chinensis attenuates glutamate-induced neurotoxicity in rat cortical cultures probably by inhibiting nitric oxide production. / Kim, So Ra; Sung, Sang Hyun; Kang, So Young; Koo, Kyung Ah; Kim, Seung Hyun; Ma, Choong Je; Lee, Heum Sook; Park, Mi Jung; Kim, Young Choong.

In: Planta Medica, Vol. 70, No. 5, 01.05.2004, p. 391-396.

Research output: Contribution to journalArticle

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