Aromatic hydroxylation of indan by o-xylene-degrading rhodococcus sp. Strain DK17

Dockyu Kim, Choong Hwan Lee, Jung Nam Choi, Ki Young Choi, Gerben J. Zylstra, Eungbin KIm

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o-xylene pathway. Metabolite and reverse transcription-PCR analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form av-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-Indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.

Original languageEnglish
Pages (from-to)375-377
Number of pages3
JournalApplied and Environmental Microbiology
Volume76
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

Rhodococcus
xylene
hydroxylation
Hydroxylation
cleavage
aromatic compounds
Dioxygenases
metabolite
substrate
Reverse Transcription
Oxidoreductases
reverse transcriptase polymerase chain reaction
metabolites
Polymerase Chain Reaction
Growth
2-xylene
indan
indans

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Kim, Dockyu ; Lee, Choong Hwan ; Choi, Jung Nam ; Choi, Ki Young ; Zylstra, Gerben J. ; KIm, Eungbin. / Aromatic hydroxylation of indan by o-xylene-degrading rhodococcus sp. Strain DK17. In: Applied and Environmental Microbiology. 2010 ; Vol. 76, No. 1. pp. 375-377.
@article{c16765a913184bebb48f3ae3a576a5c7,
title = "Aromatic hydroxylation of indan by o-xylene-degrading rhodococcus sp. Strain DK17",
abstract = "The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o-xylene pathway. Metabolite and reverse transcription-PCR analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form av-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-Indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.",
author = "Dockyu Kim and Lee, {Choong Hwan} and Choi, {Jung Nam} and Choi, {Ki Young} and Zylstra, {Gerben J.} and Eungbin KIm",
year = "2010",
month = "1",
day = "1",
doi = "10.1128/AEM.01745-09",
language = "English",
volume = "76",
pages = "375--377",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "1",

}

Aromatic hydroxylation of indan by o-xylene-degrading rhodococcus sp. Strain DK17. / Kim, Dockyu; Lee, Choong Hwan; Choi, Jung Nam; Choi, Ki Young; Zylstra, Gerben J.; KIm, Eungbin.

In: Applied and Environmental Microbiology, Vol. 76, No. 1, 01.01.2010, p. 375-377.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Aromatic hydroxylation of indan by o-xylene-degrading rhodococcus sp. Strain DK17

AU - Kim, Dockyu

AU - Lee, Choong Hwan

AU - Choi, Jung Nam

AU - Choi, Ki Young

AU - Zylstra, Gerben J.

AU - KIm, Eungbin

PY - 2010/1/1

Y1 - 2010/1/1

N2 - The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o-xylene pathway. Metabolite and reverse transcription-PCR analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form av-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-Indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.

AB - The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o-xylene pathway. Metabolite and reverse transcription-PCR analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form av-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-Indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.

UR - http://www.scopus.com/inward/record.url?scp=76149089106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76149089106&partnerID=8YFLogxK

U2 - 10.1128/AEM.01745-09

DO - 10.1128/AEM.01745-09

M3 - Article

VL - 76

SP - 375

EP - 377

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 1

ER -