Asiatic acid derivatives protect primary cultures of rat hepatocytes against carbon tetrachloride-induced injury via the cellular antioxidant system

Mi Kyeong Lee, Seung Hyun Kim, Hyekyung Yang, Doo Yeon Lim, Je Ho Ryu, Eung Seok Lee, Sang Sup Jew, Hyeung Guen Park, Sang Hyun Sung, Young Choong Kim

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Abstract

We attempted to elucidate the hepatoprotective mechanism of two asiatic acid (AS) derivatives, 3β,23-dihydroxyurs-2-oxo-12-ene-28-oic acid (AS-10) and 3β,23-dihydroxyurs-12-ene-28-oic acid (AS-14), which exhibited significant protective activity against carbon tetrachloride (CCl 4)-induced hepatotoxicity in primary cultures of rat hepatocytes. Our findings showed that AS-10 and AS-14 preserved the level of glutathione and the activities of antioxidant enzymes such as glutathione reductase, glutathione peroxidase, superoxide dismutase and catalase. In addition, these compounds ameliorated lipid peroxidation, as demonstrated by a reduction in the production of malondialdehyde. Furthermore, AS-10 and AS-14 did not restore the reduced total GSH level by BSO, indicating that the hepatoprotective activities of these compounds may be involved, in part, by regulating GSH synthesis. From these results, we suggest that both AS-10 and AS-14 exerted their hepatoprotective activities against CCl4-induced injury by preserving the cellular antioxidative defense system.

Original languageEnglish
Pages (from-to)765-768
Number of pages4
JournalNatural Product Communications
Volume4
Issue number6
Publication statusPublished - 2009 Dec 1

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery
  • Plant Science
  • Complementary and alternative medicine

Cite this

Lee, M. K., Kim, S. H., Yang, H., Lim, D. Y., Ryu, J. H., Lee, E. S., Jew, S. S., Park, H. G., Sung, S. H., & Kim, Y. C. (2009). Asiatic acid derivatives protect primary cultures of rat hepatocytes against carbon tetrachloride-induced injury via the cellular antioxidant system. Natural Product Communications, 4(6), 765-768.